Inclusion of abandoned rhubarb stalk enhanced anaerobic fermentation of alfalfa on the Qinghai Tibetan Plateau

被引：29

作者：

Li, Ping ^{[1
,2
]}

Zhao, Wenji ^{[2
]}

Yan, Lijun ^{[2
]}

Chen, Liangyin ^{[1
,2
]}

Chen, Yulian ^{[1
]}

Gou, Wenlong ^{[2
]}

You, Minghong ^{[2
]}

Cheng, Qiming ^{[1
]}

Chen, Chao ^{[1
]}

机构：

[1] Guizhou Univ, Coll Anim Sci, Guiyang, Peoples R China

[2] Sichuan Acad Grassland Sci, Chengdu 611431, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 347卷

关键词：

Rhubarb by-products; Lactobacillus species; Silage fermentation; Next generation sequencing; Alfalfa; FORAGE CROPS; SILAGE; DIVERSITY; INOCULANT; COMMUNITY; ACIDS; SPP;

D O I：

10.1016/j.biortech.2021.126347

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

To investigate the effects of lactic acid bacteria inoculant (LI) and abandoned rhubarb stalk (RS) on the anaerobic fermentation and bacterial community of alfalfa on the Qinghai Tibetan Plateau, the alfalfa was harvested and ensiled without (control) or with LI and RS at ambient temperature (5 ~ 15celcius) for 90 days. Addition of RS at ensiling increased (P < 0.05) lactate, acetate and propionate contents, and decreased (P < 0.05) the final pH value as compared with control. Addition of RS increased (P < 0.05) the bacterial alpha diversity indices, while inherent Lactococcus lactis and/or Lactobacillus sakei dominated the anaerobic fermentation. In particular, addition of RS restricted the growth of yeasts and Lactobacillales at the early stage of ensiling, but continuously stimulated anaerobic fermentation. These indicates that RS could be used as additive to facilitate anaerobic fermentation of alfalfa.

引用

页数：9

共 34 条

[1]

[Anonymous], 1991, The Biochemistry of Silage

[2]

AOAC, 1990, Official methods of analysis, V15

[3] Dark fermentative biohydrogen production from synthetic cheese whey in an anaerobic structured-bed reactor: Performance evaluation and kinetic modeling [J].

Blanco, V. M. C. ;

Oliveira, G. H. D. ;

Zaiat, M. .

RENEWABLE ENERGY, 2019, 139 :1310-1319

[4] AUTOMATED SIMULTANEOUS DETERMINATION OF AMMONIA AND TOTAL AMINO-ACIDS IN RUMINAL FLUID AND INVITRO MEDIA [J].

BRODERICK, GA ;

KANG, JH .

JOURNAL OF DAIRY SCIENCE, 1980, 63 (01) :64-75

[5] Exploring the combined effect of total ammonia nitrogen, pH and temperature on anaerobic digestion of chicken manure using response surface methodology and two kinetic models [J].

Cai, Yafan ;

Gallegos, Daniela ;

Zheng, Zehui ;

Stinner, Walter ;

Wang, Xiaofen ;

Proter, Jurgen ;

Schaefer, Franziska .

BIORESOURCE TECHNOLOGY, 2021, 337

[6]

Cai YM, 1998, APPL ENVIRON MICROB, V64, P2982

[7] Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage [J].

Cai, YM ;

Benno, Y ;

Ogawa, M ;

Kumai, S .

JOURNAL OF DAIRY SCIENCE, 1999, 82 (03) :520-526

[8] Effect of wet sea buckthorn pomace utilized as an additive on silage fermentation profile and bacterial community composition of alfalfa [J].

Chen, Liangyin ;

Qu, Hui ;

Bai, Shiqie ;

Yan, Lijun ;

You, Minghong ;

Gou, Wenlong ;

Li, Ping ;

Gao, Fengqin .

BIORESOURCE TECHNOLOGY, 2020, 314

[9]

Clementi EM, 2010, BIOACTIVE FOODS IN PROMOTING HEALTH: FRUITS AND VEGETABLES, P407, DOI 10.1016/B978-0-12-374628-3.00027-X

[10] Decrease of ruminal methane production in Rusitec fermenters through the addition of plant material from rhubarb (Rheum spp.) and alder buckthorn (Frangula alnus) [J].

Garcia-Gonzalez, R. ;

Gonzalez, J. S. ;

Lopez, S. .

JOURNAL OF DAIRY SCIENCE, 2010, 93 (08) :3755-3763

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