A novel photoacoustic-fluorescent contrast agent for quantitative imaging of lymphatic drainage

被引：16

作者：

Cardinell, Kirsten ^{[1
,2
,3
]}

Gupta, Neeru ^{[1
,2
,4
,7
,8
]}

Koivisto, Bryan D. ^{[9
]}

Kumaradas, J. Carl ^{[3
]}

Zhou, Xun ^{[1
,2
]}

Irving, Hyacinth ^{[1
]}

Luciani, Paola ^{[10
]}

Yucel, Yeni H. ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Unity Hlth Toronto, St Michaels Hosp, Li Ka Shing Knowledge Inst, Keenan Res Ctr Biomed Sci, Toronto, ON, Canada

[2] Univ Toronto, Unity Hlth Toronto, St Michaels Hosp, Dept Ophthalmol & Vis Sci, Toronto, ON, Canada

[3] Ryerson Univ, Fac Sci, Dept Phys, Toronto, ON, Canada

[4] Univ Toronto, St Michaels Hosp, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[5] Ryerson Univ, St Michaels Hosp, Inst Biomed Engn Sci & Technol iBEST, Toronto, ON, Canada

[6] Ryerson Univ, Fac Engn & Architectural Sci, Dept Mech Engn, Toronto, ON, Canada

[7] Unity Hlth Toronto, St Michaels Hosp, Glaucoma Unit, Toronto, ON, Canada

[8] Univ Toronto, Dalla Lana Sch Publ Hlth, Toronto, ON, Canada

[9] Ryerson Univ, Dept Biol & Chem, Toronto, ON, Canada

[10] Univ Bern, Dept Chem Biochem & Pharmaceut Sci, Freiestr 3, CH-3012 Bern, Switzerland

来源：

PHOTOACOUSTICS | 2021年 / 21卷

基金：

加拿大健康研究院; 加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

Contrast agent; Minimum detectable concentration; Limit of detection; Linear range; Photobleaching; Photostability; Fluorescence imaging; Lymphatic imaging; Near-infrared; NODE; EYE;

D O I：

10.1016/j.pacs.2021.100239

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

In vivo near-infrared (NIR) photoacoustic imaging (PAI) studies using novel contrast agents require validation, often via fluorescence imaging. Bioconjugation of NIR dyes to proteins is a versatile platform to obtain contrast agents for specific biomedical applications. Nonfluorescent NIR dyes with higher photostability present advantages for quantitative PAI, compared to most fluorescent NIR dyes. However, they don't provide a fluorescence signal required for fluorescence imaging. Here, we designed a hybrid PA-fluorescent contrast agent by conjugating albumin with a NIR nonfluorescent dye (QC-1) and a visible spectrum fluorescent dye, a BODIPY derivative. The new hybrid tracer QC-1/BSA/BODIPY (QBB) had a low minimum detectable concentration (2.5 mu M), a steep linear range (2.4-54.4 mu M; slope 3.39 E -5), and high photostability. Tracer signal was measured in vivo using PAI to quantify its drainage from eye to the neck and its localization in the neck lymph node was validated with postmortem fluorescence imaging.

引用

页数：9

共 59 条

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