Structure of the O-polysaccharide of Escherichia coli O132

被引：14

作者：

Shashkov, Alexander S. ^{[1
]}

Zhang, Wenwen ^{[2
]}

Perepelov, Andrei V. ^{[1
]}

Weintraub, Andrej ^{[3
]}

Liu, Bin ^{[2
]}

Widmalm, Goeran ^{[4
]}

Knirel, Yuriy A. ^{[1
]}

机构：

[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China

[3] Karolinska Univ Hosp, Karolinska Inst, Div Clin Microbiol, Dept Lab Med, S-14186 Stockholm, Sweden

[4] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden

来源：

CARBOHYDRATE RESEARCH | 2016年 / 427卷

基金：

中国国家自然科学基金; 瑞典研究理事会; 俄罗斯基础研究基金会; 对外科技合作项目（国际科技项目）;

关键词：

Escherichia coli; Lipopolysaccharide; O-Polysaccharide; Bacterial polysaccharide structure; O-Antigen gene cluster; VIRULENCE GENES; L-RHAMNOSE; PURIFICATION; SEROTYPES; STRAINS; ANTIGEN; NMR;

D O I：

10.1016/j.carres.2016.03.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mild acid degradation of the lipopolysaccharide of Escherichia coli O132 released its O-polysaccharide. Analysis by 1D and 2D H-1 and C-13 NMR spectroscopy prior and subsequent to O-deacetylation, in conjunction with sugar analysis, revealed a linear pentasaccharide repeating unit of the O-polysaccharide having the following structure: -> 2)-alpha-D-Galf-(1 -> 3)-alpha-L-Rhap2Ac-(1 -> 4)-alpha-D-Glcp-(1 -> 2)-alpha-L-Rhap-(1 -> 3)-beta-D-GlcpNAc-(1 -> Putative functions of genes in the O-antigen gene cluster of E. coli O132 are consistent with the O-polysaccharide structure. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：44 / 47

页数：4

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