High concentration ethanol production from corncob residues by fed-batch strategy

被引：140

作者：

Liu, Kai ^{[1
]}

Lin, Xiaohui ^{[1
]}

Yue, Jun ^{[1
]}

Li, Xuezhi ^{[1
]}

Fang, Xu ^{[1
]}

Zhu, Mingtian ^{[1
]}

Lin, Jianqiang ^{[1
]}

Qu, Yinbo ^{[1
]}

Xiao, Lin ^{[2
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Jinan 250100, Shandong, Peoples R China

[2] Longlive Biotechnol Co Ltd, High Technol Dev Zone, Yucheng 251200, Shandong, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 13期

关键词：

Corncob; Bioethanol; Fed-batch; Simultaneous saccharification and fermentation; Penicillium decumbens; DRY-MATTER CONTENT; ENZYMATIC-HYDROLYSIS; SIMULTANEOUS SACCHARIFICATION; CELLULASE ADSORPTION; TRICHODERMA-REESEI; LIGNIN REMOVAL; STOVER; FERMENTATION; DIGESTIBILITY; PRETREATMENT;

D O I：

10.1016/j.biortech.2009.11.013

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Ethanol production from corncob residues (CCR) pretreated by different methods was studied. The structure features of these CCR were analyzed by Fourier transform-infrared spectrum (FT-IR). X-ray diffraction (XRD), and field emission scanning electron microscope (SEM). Simultaneous saccharification and fermentation (SSF) was performed by adding crude cellulase preparations from Penicillium decumbens JUA10-1 at 30 degrees C. The results suggested that different pretreatments resulted in different composition and structure of residues; these changes had a significant influence on ethanol productivity and concentration. The fed-batch method was combined with SSF to enhance ethanol concentration further and reduce enzyme dosage. Moreover, the absorption and desorption phenomena of cellobiohydrolase I (CBH 1) (70 kDa) were observed to be related to lignin contents in residues. These results demonstrated that despite the application of low enzyme dosage, high concentration ethanol could be produced from pretreated corncobs by combining fed-batch method with SSF. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4952 / 4958

页数：7

共 34 条

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