CRISPR/Cas9: From Genome Engineering to Cancer Drug Discovery

被引：40

作者：

Luo, Ji ^{[1
]}

机构：

[1] NCI, Lab Canc Biol & Genet, Ctr Canc Res, NIH, Bethesda, MD 20814 USA

来源：

TRENDS IN CANCER | 2016年 / 2卷 / 06期

关键词：

RNA-GUIDED ENDONUCLEASE; HUMAN-CELLS; CHROMOSOMAL REARRANGEMENTS; CRYSTAL-STRUCTURE; ESSENTIAL GENES; MOUSE MODEL; CRISPR-CAS9; CAS9; IDENTIFICATION; GENERATION;

D O I：

10.1016/j.trecan.2016.05.001

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Advances in translational research are often driven by new technologies. The advent of microarrays, next-generation sequencing, proteomics, and RNAi have led to breakthroughs in our understanding of the mechanisms of cancer and in the discovery of new cancer drug targets. The discovery of the bacterial clustered regularly interspaced palindromic repeat (CRISPR) system and its subsequent adaptation as a tool for mammalian genome engineering has opened new avenues for functional genomics studies. This review focuses on the utility of CRISPR in the context of cancer drug target discovery.

引用

页码：313 / 324

页数：12

共 73 条

[1] Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease [J].

Anders, Carolin ;

Niewoehner, Ole ;

Duerst, Alessia ;

Jinek, Martin .

NATURE, 2014, 513 (7519) :569-+

[2] The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity [J].

Barretina, Jordi ;

Caponigro, Giordano ;

Stransky, Nicolas ;

Venkatesan, Kavitha ;

Margolin, Adam A. ;

Kim, Sungjoon ;

Wilson, Christopher J. ;

Lehar, Joseph ;

Kryukov, Gregory V. ;

Sonkin, Dmitriy ;

Reddy, Anupama ;

Liu, Manway ;

Murray, Lauren ;

Berger, Michael F. ;

Monahan, John E. ;

Morais, Paula ;

Meltzer, Jodi ;

Korejwa, Adam ;

Jane-Valbuena, Judit ;

Mapa, Felipa A. ;

Thibault, Joseph ;

Bric-Furlong, Eva ;

Raman, Pichai ;

Shipway, Aaron ;

Engels, Ingo H. ;

Cheng, Jill ;

Yu, Guoying K. ;

Yu, Jianjun ;

Aspesi, Peter, Jr. ;

de Silva, Melanie ;

Jagtap, Kalpana ;

Jones, Michael D. ;

Wang, Li ;

Hatton, Charles ;

Palescandolo, Emanuele ;

Gupta, Supriya ;

Mahan, Scott ;

Sougnez, Carrie ;

Onofrio, Robert C. ;

Liefeld, Ted ;

MacConaill, Laura ;

Winckler, Wendy ;

Reich, Michael ;

Li, Nanxin ;

Mesirov, Jill P. ;

Gabriel, Stacey B. ;

Getz, Gad ;

Ardlie, Kristin ;

Chan, Vivien ;

Myer, Vic E. .

NATURE, 2012, 483 (7391) :603-607

[3]

Bassik MC, 2009, NAT METHODS, V6, P443, DOI [10.1038/NMETH.1330, 10.1038/nmeth.1330]

[4] Simple and Rapid In Vivo Generation of Chromosomal Rearrangements using CRISPR/Cas9 Technology [J].

Blasco, Rafael B. ;

Karaca, Elif ;

Ambrogio, Chiara ;

Cheong, Taek-Chin ;

Karayol, Emre ;

Minero, Valerio G. ;

Voena, Claudia ;

Chiarle, Roberto .

CELL REPORTS, 2014, 9 (04) :1219-1227

[5] Global gene disruption in human cells to assign genes to phenotypes by deep sequencing [J].

Carette, Jan E. ;

Guimaraes, Carla P. ;

Wuethrich, Irene ;

Blomen, Vincent A. ;

Varadarajan, Malini ;

Sun, Chong ;

Bell, George ;

Yuan, Bingbing ;

Muellner, Markus K. ;

Nijman, Sebastian M. ;

Ploegh, Hidde L. ;

Brummelkamp, Thijn R. .

NATURE BIOTECHNOLOGY, 2011, 29 (06) :542-U108

[6]

Chari R, 2015, NAT METHODS, V12, P823, DOI [10.1038/NMETH.3473, 10.1038/nmeth.3473]

[7] Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer [J].

Cheung, Hiu Wing ;

Cowley, Glenn S. ;

Weir, Barbara A. ;

Boehm, Jesse S. ;

Rusin, Scott ;

Scott, Justine A. ;

East, Alexandra ;

Ali, Levi D. ;

Lizotte, Patrick H. ;

Wong, Terence C. ;

Jiang, Guozhi ;

Hsiao, Jessica ;

Mermel, Craig H. ;

Getz, Gad ;

Barretina, Jordi ;

Gopal, Shuba ;

Tamayo, Pablo ;

Gould, Joshua ;

Tsherniak, Aviad ;

Stransky, Nicolas ;

Luo, Biao ;

Ren, Yin ;

Drapkin, Ronny ;

Bhatia, Sangeeta N. ;

Mesirov, Jill P. ;

Garraway, Levi A. ;

Meyerson, Matthew ;

Lander, Eric S. ;

Root, David E. ;

Hahn, William C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (30) :12372-12377

[8] Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease [J].

Cho, Seung Woo ;

Kim, Sojung ;

Kim, Jong Min ;

Kim, Jin-Soo .

NATURE BIOTECHNOLOGY, 2013, 31 (03) :230-232

[9] Targeted genomic rearrangements using CRISPR/Cas technology [J].

Choi, Peter S. ;

Meyerson, Matthew .

NATURE COMMUNICATIONS, 2014, 5

[10] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

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