Stretching of red blood cells using an electro-optics trap

被引：22

作者：

Haque, Md. Mozzammel ^{[1
,2
]}

Moisescu, Mihaela G. ^{[2
]}

Valkai, Sandor ^{[1
]}

Der, Andras ^{[1
]}

Savopol, Tudor ^{[2
]}

机构：

[1] Hungarian Acad Sci, Biol Res Ctr, Inst Biophys, H-6726 Szeged, Hungary

[2] Carol Davila Univ Med & Pharm, Dept Biophys & Cellular Biotechnol, Bucharest, Romania

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 01期

关键词：

HUMAN ERYTHROCYTE-MEMBRANE; OPTICAL TWEEZERS; DEFORMABILITY; MANIPULATION; MECHANICS; SYSTEM;

D O I：

10.1364/BOE.6.000118

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The stretching stiffness of Red Blood Cells (RBCs) was investigated using a combination of an AC dielectrophoretic apparatus and a single-beam optical tweezer. The experiments were performed at 10 MHz, a frequency high enough to avoid conductivity losses, but below the second turnover point between positive and negative dielectrophoresis. By measuring the geometrical parameters of single healthy human RBCs as a function of the applied voltage, the elastic modulus of RBCs was determined (mu = 1.80 +/- 0.5 mu N/m) and compared with similar values of the literature got by other techniques. The method is expected to be an easy-to-use, alternative tool to determine the mechano-elastic properties of living cells, and, on this basis, to distinguish healthy and diseased cells (C) 2014 Optical Society of America

引用

页码：118 / 123

页数：6

共 24 条

[11] A new determination of the shear modulus of the human erythrocyte membrane using optical tweezers [J].

Hénon, S ;

Lenormand, G ;

Richert, A ;

Gallet, F .

BIOPHYSICAL JOURNAL, 1999, 76 (02) :1145-1151

[12] Light-induced dielectrophoretic manipulation of DNA [J].

Hoeb, Marco ;

Raedler, Joachim O. ;

Klein, Stefan ;

Stutzmann, Martin ;

Brandt, Martin S. .

BIOPHYSICAL JOURNAL, 2007, 93 (03) :1032-1038

[13] Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system [J].

Hwang, Hyundoo ;

Choi, Yong-Je ;

Choi, Wonjae ;

Kim, Se-Hwan ;

Jang, Jin ;

Park, Je-Kyun .

ELECTROPHORESIS, 2008, 29 (06) :1203-1212

[14] RED CELL FILTRATION AND PATHOGENESIS OF CERTAIN HEMOLYTIC ANEMIAS [J].

JANDL, JH ;

CASTLE, WB ;

SIMMONS, RL .

BLOOD, 1961, 18 (02) :133-&

[15] Direct measurement of the area expansion and shear moduli of the human red blood cell membrane skeleton [J].

Lenormand, G ;

Hénon, S ;

Richert, A ;

Siméon, J ;

Gallet, F .

BIOPHYSICAL JOURNAL, 2001, 81 (01) :43-56

[16] REDUCED ERYTHROCYTE DEFORMABILITY IN DIABETES [J].

MCMILLAN, DE ;

UTTERBACK, NG ;

LAPUMA, J .

DIABETES, 1978, 27 (09) :895-901

[17] Measurement of the nonlinear elasticity of red blood cell membranes [J].

Park, YongKeun ;

Best, Catherine A. ;

Kuriabova, Tatiana ;

Henle, Mark L. ;

Feld, Michael S. ;

Levine, Alex J. ;

Popescu, Gabriel .

PHYSICAL REVIEW E, 2011, 83 (05)

[18] Metabolic remodeling of the human red blood cell membrane [J].

Park, YongKeun ;

Best, Catherine A. ;

Auth, Thorsten ;

Gov, Nir S. ;

Safran, Samuel A. ;

Popescu, Gabriel ;

Suresh, Subra ;

Feld, Michael S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (04) :1289-1294

[19]

Pohl H.A., 1978, DIELECTROPHORESIS

[20] Viscoelasticity of the human red blood cell [J].

Puig-De-Morales-Marinkovic, Marina ;

Turner, Kevin T. ;

Butler, James P. ;

Fredberg, Jeffrey J. ;

Suresh, Subra .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2007, 293 (02) :C597-C605

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