Baicalin inhibits autophagy induced by influenza A virus H3N2

被引：72

作者：

Zhu, Hai-yan ^{[1
]}

Han, Lei ^{[1
]}

Shi, Xun-long ^{[1
]}

Wang, Bao-long ^{[1
]}

Huang, Hai ^{[1
]}

Wang, Xin ^{[1
]}

Chen, Dao-feng ^{[2
]}

Ju, Dian-wen ^{[1
]}

Feng, Mei-qing ^{[1
]}

机构：

[1] Fudan Univ, Sch Pharm, Dept Biosynth, Shanghai 201203, Peoples R China

[2] Fudan Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 201203, Peoples R China

来源：

ANTIVIRAL RESEARCH | 2015年 / 113卷

基金：

中国国家自然科学基金;

关键词：

Influenza A virus; Autophagy; Baicalin; H3N2; NS1; PROTEIN; PATHWAY; GROWTH; IDENTIFICATION; SUPPRESSION; INFECTION; APOPTOSIS; TARGET; CELLS; AKT;

D O I：

10.1016/j.antiviral.2014.11.003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Baicalin, a natural product isolated from Scutellaria radix, has been reported to have significant in vivo and in vitro anti-influenza virus activity, but the underlying mechanism remains poorly understood. In this study, we found that baicalin inhibited autophagy induced by influenza virus A3/Beijing/30/95 (H3N2) in both A549 and Ana-1 cells. The results showed that H3N2 induced autophagy by suppressing mTOR signaling pathway, which however could be significantly inhibited by baicalin. Baicalin could suppress the expression of Atg5-Atg12 complex and LC3-II, and attenuate autophagy induced by starvation. Thus, the inhibition of autophagy induced by virus may account for the antiviral activities of baicalin against H3N2. Autophagy may be a potential marker in developing novel anti-influenza drugs. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：62 / 70

页数：9

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