Baicalin inhibits autophagy induced by influenza A virus H3N2

被引：72

作者：

Zhu, Hai-yan ^{[1
]}

Han, Lei ^{[1
]}

Shi, Xun-long ^{[1
]}

Wang, Bao-long ^{[1
]}

Huang, Hai ^{[1
]}

Wang, Xin ^{[1
]}

Chen, Dao-feng ^{[2
]}

Ju, Dian-wen ^{[1
]}

Feng, Mei-qing ^{[1
]}

机构：

[1] Fudan Univ, Sch Pharm, Dept Biosynth, Shanghai 201203, Peoples R China

[2] Fudan Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 201203, Peoples R China

来源：

ANTIVIRAL RESEARCH | 2015年 / 113卷

基金：

中国国家自然科学基金;

关键词：

Influenza A virus; Autophagy; Baicalin; H3N2; NS1; PROTEIN; PATHWAY; GROWTH; IDENTIFICATION; SUPPRESSION; INFECTION; APOPTOSIS; TARGET; CELLS; AKT;

D O I：

10.1016/j.antiviral.2014.11.003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Baicalin, a natural product isolated from Scutellaria radix, has been reported to have significant in vivo and in vitro anti-influenza virus activity, but the underlying mechanism remains poorly understood. In this study, we found that baicalin inhibited autophagy induced by influenza virus A3/Beijing/30/95 (H3N2) in both A549 and Ana-1 cells. The results showed that H3N2 induced autophagy by suppressing mTOR signaling pathway, which however could be significantly inhibited by baicalin. Baicalin could suppress the expression of Atg5-Atg12 complex and LC3-II, and attenuate autophagy induced by starvation. Thus, the inhibition of autophagy induced by virus may account for the antiviral activities of baicalin against H3N2. Autophagy may be a potential marker in developing novel anti-influenza drugs. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：62 / 70

页数：9

共 37 条

[1] Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand [J].

Bai, Gui-Rong ;

Chittaganpitch, Malinee ;

Kanai, Yuta ;

Li, Yong-Gang ;

Auwanit, Wattana ;

Ikuta, Kazuyoshi ;

Sawanpanyalert, Pathom .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 390 (03) :897-901

[2] Acute respiratory distress syndrome and pneumonia:: A comprehensive review of clinical data [J].

Bauer, Torsten T. ;

Ewig, Santiago ;

Rodloff, Arne C. ;

Mueller, Eckhard E. .

CLINICAL INFECTIOUS DISEASES, 2006, 43 (06) :748-756

[3] Current and future antiviral therapy of severe seasonal and avian influenza [J].

Beigel, John ;

Bray, Mike .

ANTIVIRAL RESEARCH, 2008, 78 (01) :91-102

[4] The mechanism of action of ribavirin: lethal mutagenesis of RNA virus genomes mediated by the viral RNA-dependent RNA polymerase [J].

Cameron, CE ;

Castro, C .

CURRENT OPINION IN INFECTIOUS DISEASES, 2001, 14 (06) :757-764

[5] A novel image-based cytometry method for autophagy detection in living cells [J].

Chan, Leo Li-Ying ;

Shen, Dee ;

Wilkinson, Alisha R. ;

Patton, Wayne ;

Lai, Ning ;

Chan, Eric ;

Kuksin, Dmitry ;

Lin, Bo ;

Qiu, Jean .

AUTOPHAGY, 2012, 8 (09) :1371-1382

[6] Activity of the Novel Dual Phosphatidylinositol 3-Kinase/Mammalian Target of Rapamycin Inhibitor NVP-BEZ235 against T-Cell Acute Lymphoblastic Leukemia [J].

Chiarini, Francesca ;

Grimaldi, Cecilia ;

Ricci, Francesca ;

Tazzari, Pier Luigi ;

Evangelisti, Camilla ;

Ognibene, Andrea ;

Battistelli, Michela ;

Falcieri, Elisabetta ;

Melchionda, Fraia ;

Pession, Andrea ;

Pagliaro, Pasqualepaolo ;

McCubrey, James A. ;

Martelli, Alberto M. .

CANCER RESEARCH, 2010, 70 (20) :8097-8107

[7]

Chu Zheng-yun, 2007, Zhongguo Zhong Yao Za Zhi, V32, P2413

[8] Functional Macroautophagy Induction by Influenza A Virus without a Contribution to Major Histocompatibility Complex Class II-Restricted Presentation [J].

Comber, Joseph D. ;

Robinson, Tara M. ;

Siciliano, Nicholas A. ;

Snook, Adam E. ;

Eisenlohr, Laurence C. .

JOURNAL OF VIROLOGY, 2011, 85 (13) :6453-6463

[9] Identification of 23-(S)-2-Amino-3-Phenylpropanoyl-Silybin as an Antiviral Agent for Influenza A Virus Infection In Vitro and In Vivo [J].

Dai, Jian-Ping ;

Wu, Li-Qi ;

Li, Rui ;

Zhao, Xiang-Feng ;

Wan, Qian-Ying ;

Chen, Xiao-Xuan ;

Li, Wei-Zhong ;

Wang, Ge-Fei ;

Li, Kang-Sheng .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (09) :4433-4443

[10] A Drug Screening Method Based on the Autophagy Pathway and Studies of the Mechanism of Evodiamine against Influenza A Virus [J].

Dai, Jian-Ping ;

Li, Wei-Zhong ;

Zhao, Xiang-Feng ;

Wang, Ge-Fei ;

Yang, Jia-Cai ;

Zhang, Lin ;

Chen, Xiao-Xuan ;

Xu, Yan-Xuan ;

Li, Kang-Sheng .

PLOS ONE, 2012, 7 (08)

← 1 2 3 4 →