Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential

被引:30
作者
Martino, Nicola A. [1 ,2 ]
Dell'Aquila, Maria E. [1 ,2 ]
Uranio, Manuel Filioli [1 ,2 ]
Rutigliano, Lucia [1 ,2 ]
Nicassio, Michele [1 ,2 ]
Lacalandra, Giovanni M. [1 ,2 ]
Hinrichs, Katrin [3 ,4 ]
机构
[1] Univ Bari Aldo Moro, Vet Clin, I-70010 Bari, Italy
[2] Univ Bari Aldo Moro, Anim Prod Unit, Dipartimento Emergenza & Trapianti DOrgano DETO, I-70010 Bari, Italy
[3] Texas A&M Univ, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA
[4] Texas A&M Univ, Dept Large Anim Clin Sci, College Stn, TX 77843 USA
来源
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY | 2014年 / 12卷
关键词
Pre-maturational oocyte holding; Nuclear chromatin configuration; Germinal vesicle; Mitochondria; Reactive oxygen species; INTRACYTOPLASMIC SPERM INJECTION; INITIAL CHROMATIN CONFIGURATION; CELL NUCLEAR TRANSFER; BLASTOCYST DEVELOPMENT; CUMULUS MORPHOLOGY; HORSE OOCYTES; PRONUCLEUS FORMATION; GERMINAL VESICLE; BOVINE OOCYTES; COMPETENCE;
D O I
10.1186/1477-7827-12-99
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Evaluation of mitochondrial function offers an alternative to evaluate embryo development for assessment of oocyte viability, but little information is available on the relationship between mitochondrial and chromatin status in equine oocytes. We evaluated these parameters in immature equine oocytes either fixed immediately (IMM) or held overnight in an Earle's/Hank's' M199-based medium in the absence of meiotic inhibitors (EH treatment), and in mature oocytes. We hypothesized that EH holding may affect mitochondrial function and that holding temperature may affect the efficiency of meiotic suppression. Methods: Experiment 1 -Equine oocytes processed immediately or held in EH at uncontrolled temperature (22 to 27 degrees C) were evaluated for initial chromatin configuration, in vitro maturation (IVM) rates and mitochondrial energy/redox potential. Experiment 2 -We then investigated the effect of holding temperature (25 degrees C, 30 degrees C, 38 degrees C) on initial chromatin status of held oocytes, and subsequently repeated mitochondrial energy/redox assessment of oocytes held at 25 degrees C vs. immediately-evaluated controls. Results: EH holding at uncontrolled temperature was associated with advancement of germinal vesicle (GV) chromatin condensation and with meiotic resumption, as well as a lower maturation rate after IVM. Holding did not have a significant effect on mitochondrial distribution within chromatin configurations. Independent of treatment, oocytes having condensed chromatin had a significantly higher proportion of perinuclear/pericortical mitochondrial distribution than did other GV configurations. Holding did not detrimentally affect oocyte energy/redox parameters in viable GV-stage oocytes. There were no significant differences in chromatin configuration between oocytes held at 25 degrees C and controls, whereas holding at higher temperature was associated with meiosis resumption and loss of oocytes having the condensed chromatin GV configuration. Holding at 25 degrees C was not associated with progression of mitochondrial distribution pattern and there were no significant differences in oocyte energy/redox parameters between these oocytes and controls. Conclusions: Mitochondrial distribution in equine GV-stage oocytes is correlated with chromatin configuration within the GV. Progression of chromatin configuration and mitochondrial status during holding are dependent on temperature. EH holding at 25 degrees C maintains meiotic arrest, viability and mitochondrial potential of equine oocytes. This is the first report on the effects of EH treatment on oocyte mitochondrial energy/redox potential.
引用
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页数:12
相关论文
共 48 条
[1]   Holding bovine oocytes in the absence of maturation inhibitors: Kinetics of in vitro maturation and effect on blastocyst development after in vitro fertilization [J].
Alm, H. ;
Choi, Y. H. ;
Love, L. ;
Heleil, B. ;
Torner, H. ;
Hinrichs, K. .
THERIOGENOLOGY, 2008, 70 (07) :1024-1029
[2]   In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems [J].
Alonso, A. ;
Castex, C. Baca ;
Ferrante, A. ;
Pinto, M. ;
Castaneira, C. ;
Trasorras, V. ;
Gambarotta, M. C. ;
Losinno, L. ;
Miragaya, M. .
ANDROLOGIA, 2015, 47 (04) :387-394
[3]   Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI [J].
Ambruosi, B. ;
Lacalandra, G. M. ;
Lorga, A. I. ;
De Santis, T. ;
Mugnier, S. ;
Matarrese, R. ;
Goudet, G. ;
Dell'Aquila, M. E. .
THERIOGENOLOGY, 2009, 71 (07) :1093-1104
[4]   In Vitro Acute Exposure to DEHP Affects Oocyte Meiotic Maturation, Energy and Oxidative Stress Parameters in a Large Animal Model [J].
Ambruosi, Barbara ;
Uranio, Manuel Filioli ;
Sardanelli, Anna Maria ;
Pocar, Paola ;
Martino, Nicola Antonio ;
Paternoster, Maria Stefania ;
Amati, Francesca ;
Dell'Aquila, Maria Elena .
PLOS ONE, 2011, 6 (11)
[5]   Adult Body Weight Is Programmed by a Redox-Regulated and Energy-Dependent Process during the Pronuclear Stage in Mouse [J].
Banrezes, Bernadette ;
Sainte-Beuve, Thierry ;
Canon, Eugenie ;
Schultz, Richard M. ;
Cancela, Jose ;
Ozil, Jean-Pierre .
PLOS ONE, 2011, 6 (12)
[6]   Effect of holding technique and culture drop size in individual or group culture on blastocyst development after ICSI of equine oocytes with low meiotic competence [J].
Choi, Y. H. ;
Love, L. B. ;
Varner, D. D. ;
Hinrichs, K. .
ANIMAL REPRODUCTION SCIENCE, 2007, 102 (1-2) :38-47
[7]   Holding immature equine oocytes in the absence of meiotic inhibitors: Effect on germinal vesicle chromatin and blastocyst development after intracytoplasmic sperm injection [J].
Choi, Y. H. ;
Love, L. B. ;
Varner, D. D. ;
Hinrichs, K. .
THERIOGENOLOGY, 2006, 66 (04) :955-963
[8]   Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse [J].
Choi, Y. H. ;
Varner, D. D. ;
Love, C. C. ;
Hartman, D. L. ;
Hinrichs, K. .
REPRODUCTION, 2011, 142 (04) :529-538
[9]   Equine blastocyst development after intracytoplasmic injection of sperm subjected to two freeze-thaw cycles [J].
Choi, YH ;
Love, CC ;
Varner, DD ;
Hinrichs, K .
THERIOGENOLOGY, 2006, 65 (04) :808-819
[10]   Blastocyst formation rates in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection [J].
Choi, YH ;
Roasa, LM ;
Love, CC ;
Varner, DD ;
Brinsko, S ;
Hinrichs, K .
BIOLOGY OF REPRODUCTION, 2004, 70 (05) :1231-1238