Virucidal Activity of the Pyridobenzothiazolone Derivative HeE1-17Y against Enveloped RNA Viruses

被引：6

作者：

Bonotto, Rafaela Milan ^{[1
]}

Boni, Francesco ^{[2
,3
]}

Milani, Mario ^{[2
,3
]}

Chaves-Sanjuan, Antonio ^{[3
,4
]}

Franze, Silvia ^{[5
]}

Selmin, Francesca ^{[5
]}

Felicetti, Tommaso ^{[6
]}

Bolognesi, Martino ^{[3
,4
]}

Konstantinidou, Soultana ^{[1
]}

Poggianella, Monica ^{[1
]}

Marquez, Chantal L. ^{[1
]}

Dattola, Federica ^{[1
]}

Zoppe, Monica ^{[2
,3
]}

Manfroni, Giuseppe ^{[6
]}

Mastrangelo, Eloise ^{[2
,3
]}

Marcello, Alessandro ^{[1
]}

机构：

[1] Int Ctr Genet Engn & Biotechnol, Lab Mol Virol, Padriciano 99, I-34149 Trieste, Italy

[2] CNR IBF, Biophys Inst, Via Celoria 26, I-20133 Milan, Italy

[3] Univ Milan, Dipartiment Biosci, Via Celoria 26, I-20133 Milan, Italy

[4] Univ Milan, Fdn Romeo & Enr Invernizzi, Via Celoria 26, I-20133 Milan, Italy

[5] Univ Milan, Dept Pharmaceut Sci, Via Giuseppe Colombo 71, I-20133 Milan, Italy

[6] Univ Perugia, Dept Pharmaceut Sci, Via Fabretti 48, I-06123 Perugia, Italy

来源：

VIRUSES-BASEL | 2022年 / 14卷 / 06期

关键词：

antivirals; enveloped viruses; flavivirus; virucidal effect; reporter replicon particles; WEST-NILE-VIRUS; ANTIVIRAL ACTIVITY; ENTRY; POLYMERASE;

D O I：

10.3390/v14061157

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pyridobenzothiazolone derivatives are a promising class of broad-spectrum antivirals. However, the mode of action of these compounds remains poorly understood. The HeE1-17Y derivative has already been shown to be a potent compound against a variety of flaviviruses of global relevance. In this work, the mode of action of HeE1-17Y has been studied for West Nile virus taking advantage of reporter replication particles (RRPs). Viral infectivity was drastically reduced by incubating the compound with the virus before infection, thus suggesting a direct interaction with the viral particles. Indeed, RRPs incubated with the inhibitor appeared to be severely compromised in electron microscopy analysis. HeE1-17Y is active against other enveloped viruses, including SARS-CoV-2, but not against two non-enveloped viruses, suggesting a virucidal mechanism that involves the alteration of the viral membrane.

引用

页数：14

共 41 条

[41] New tools for automated high-resolution cryo-EM structure determination in RELION-3
Zivanov, Jasenko
Nakane, Takanori
Forsberg, Bjorn O.
Kimanius, Dari
Hagen, Wim J. H.
Lindahl, Erik
Scheres, Sjors H. W.
[J]. ELIFE, 2018, 7

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