Extraction of Betanin Using Aqueous Two-Phase Systems

被引:13
作者
Ebrahimi, Tahereh [1 ]
Shahriari, Shahla [2 ]
机构
[1] Islamic Azad Univ, Shahr E Qods Branch, Dept Food Sci & Technol, Tehran, Iran
[2] Islamic Azad Univ, Shahr E Qods Branch, Dept Chem Engn, Tehran, Iran
关键词
BIPHASIC SYSTEMS; POLY(ETHYLENE GLYCOL); ANTIOXIDANT ACTIVITY; IONIC LIQUIDS; PURIFICATION; PARTITION; ANTHOCYANINS; BETAXANTHINS; BETALAINS; PROTEINS;
D O I
10.1246/bcsj.20160011
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The extraction efficiency of betanin as a natural pigment with antioxidant features is significant. The main reason for studying the different aqueous two-phase systems (ATPSs) for betanin extraction is that the selection of an optimum process of separation is possible. In order to develop an efficient method for the extraction of betanin, ATPS composed of polymer and sodium sulfate was investigated. Sodium sulfate is a strong salting-out agent, which has an important effect on the processes of the extraction of biomolecules. The effect of parameters such as temperature, salt and polymer concentrations, and the molecular weight of the polymer was evaluated for the partitioning of betanin. The experimental results showed that the polymer concentrations in ATPS can significantly affect the partitioning of betanin between the top and bottom phases. The recovery percentage of betanin indicated that this system had the ability to improve the betanin recovery in the top phase.
引用
收藏
页码:565 / 572
页数:8
相关论文
共 34 条
[1]   Antioxidant activity of betalains from plants of the Amaranthaceae [J].
Cai, YZ ;
Sun, M ;
Corke, H .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (08) :2288-2294
[2]   Differential partitioning of betacyanins and betaxanthins employing aqueous two phase extraction [J].
Chandrasekhar, J. ;
Sonika, G. ;
Madhusudhan, M. C. ;
Raghavarao, K. S. M. S. .
JOURNAL OF FOOD ENGINEERING, 2015, 144 :156-163
[3]   Aqueous two phase extraction for purification and concentration of betalains [J].
Chethana, S. ;
Nayak, Chetan A. ;
Raghavarao, K. S. M. S. .
JOURNAL OF FOOD ENGINEERING, 2007, 81 (04) :679-687
[4]  
Chethana S., 2003, INDIAN PATENT, Patent No. 388/DEL/03
[5]   Extraction of vanillin using ionic-liquid-based aqueous two-phase systems [J].
Cláudio A.F.M. ;
Freire M.G. ;
Freire C.S.R. ;
Silvestre A.J.D. ;
Coutinho J.A.P. .
Separation and Purification Technology, 2010, 75 (01) :39-47
[6]   Natural pigments:: Carotenoids, anthocyanins, and betalains -: Characteristics, biosynthesis, processing, and stability [J].
Delgado-Vargas, F ;
Jiménez, AR ;
Paredes-López, O .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2000, 40 (03) :173-289
[7]   INTERFACIAL-TENSION OF POLYETHYLENEGLYCOL-DEXTRAN-WATER SYSTEMS - INFLUENCE OF TEMPERATURE AND POLYMER MOLECULAR-WEIGHT [J].
FORCINITI, D ;
HALL, CK ;
KULA, MR .
JOURNAL OF BIOTECHNOLOGY, 1990, 16 (3-4) :279-296
[8]  
Forciniti D, 2000, METH BIOTEC, V11, P23
[9]   Aqueous biphasic systems: a boost brought about by using ionic liquids [J].
Freire, Mara G. ;
Claudio, Ana Filipa M. ;
Araujo, Joao M. M. ;
Coutinho, Joao A. P. ;
Marrucho, Isabel M. ;
Canongia Lopes, Jose N. ;
Rebelo, Luis Paulo N. .
CHEMICAL SOCIETY REVIEWS, 2012, 41 (14) :4966-4995
[10]   Insight into the Interactions That Control the Phase Behaviour of New Aqueous Biphasic Systems Composed of Polyethylene Glycol Polymers and Ionic Liquids [J].
Freire, Mara G. ;
Pereira, Jorge F. B. ;
Francisco, Maria ;
Rodriguez, Hector ;
Rebelo, Luis Paulo N. ;
Rogers, Robin D. ;
Coutinho, Joao A. P. .
CHEMISTRY-A EUROPEAN JOURNAL, 2012, 18 (06) :1831-1839