FlgM Is Secreted by the Flagellar Export Apparatus in Bacillus subtilis

被引:48
作者
Calvo, Rebecca A. [1 ]
Kearns, Daniel B. [1 ]
机构
[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
关键词
ANTI-SIGMA FACTOR; SALMONELLA-TYPHIMURIUM; GENE-EXPRESSION; POPULATION HETEROGENEITY; DELETION MUTATION; ESCHERICHIA-COLI; SERINE-PROTEASE; STRUCTURAL GENE; RNA-POLYMERASE; OPERON;
D O I
10.1128/JB.02324-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The bacterial flagellum is assembled from over 20 structural components, and flagellar gene regulation is morphogenetically coupled to the assembly state by control of the anti-sigma factor FlgM. In the Gram-negative bacterium Salmonella enterica, FlgM inhibits late-class flagellar gene expression until the hook-basal body structural intermediate is completed and FlgM is inhibited by secretion from the cytoplasm. Here we demonstrate that FlgM is also secreted in the Gram-positive bacterium Bacillus subtilis and is degraded extracellularly by the proteases Epr and WprA. We further demonstrate that, like in S. enterica, the structural genes required for the flagellar hook-basal body are required for robust activation of sigma(D)-dependent gene expression and efficient secretion of FlgM. Finally, we determine that FlgM secretion is strongly enhanced by, but does not strictly require, hook-basal body completion and instead demands a minimal subset of flagellar proteins that includes the FliF/FliG basal body proteins, the flagellar type III export apparatus components FliO, FliP, FliQ, FliR, FlhA, and FlhB, and the substrate specificity switch regulator FliK.
引用
收藏
页码:81 / 91
页数:11
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