Interaction of reducible polypeptide gene delivery vectors with supported lipid bilayers: pore formation and structure-function relationships

被引：2

作者：

Soliman, Mahmoud ^{[1
,4
]}

Nasanit, Rujikan ^{[2
]}

Allen, Stephanie ^{[1
]}

Davies, Martyn C. ^{[1
]}

Briggs, Simon S. ^{[3
]}

Seymour, Leonard W. ^{[3
]}

Preece, Jon A. ^{[2
]}

Alexander, Cameron ^{[1
]}

机构：

[1] Univ Nottingham, Sch Pharm, Nottingham NG7 2RD, England

[2] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England

[3] Univ Oxford, Dept Clin Pharmacol, Oxford OX2 6HE, England

[4] Ain Shams Univ, Dept Pharmaceut, Fac Pharm, Cairo, Egypt

来源：

SOFT MATTER | 2010年 / 6卷 / 11期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

ATOMIC-FORCE MICROSCOPY; IN-VIVO; PLASMID DNA; PEPTIDES; CHOLESTEROL; DESIGN; PHOSPHATIDYLCHOLINE; OLIGONUCLEOTIDES; MEMBRANES; POLYMERS;

D O I：

10.1039/b927204f

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Real-time atomic force microscopy (AFM) in aqueous buffer has been used to probe interactions of synthetic disulfide-linked polypeptide gene delivery vectors with supported phospholipid bilayers. Disruption of the membranes was apparent in AFM, and the extent of surface heterogeneity and hole (pore) formation was evaluated by depth and area-profiling image analysis. The overall extent of membrane disruption varied with the reducible polycation peptide sequence and block structure and was found to be correlated with the overall degree of transgene expression in two representative cell lines.

引用

页码：2517 / 2524

页数：8

共 39 条

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