Efficiency of RAPD, ISSR and ITS markers in detecting genetic variability among Salacia species sampled from the Western Ghats of Karnataka

被引：3

作者：

Bajpe, Shrisha Naik ^{[1
]}

Bharathi, Tumkur Ramasetty ^{[1
]}

Marulasiddaswamy, Kuppur Mallikarjunaiah ^{[1
]}

Kumara, Kigga Kadappa Sampath ^{[2
]}

Prakash, Harishchandra Sripathy ^{[1
]}

Kini, Ramachandra Kukkundoor ^{[1
]}

机构：

[1] Univ Mysore, Dept Studies Biotechnol, Mysore 570006, Karnataka, India

[2] Govt Preuniv Coll, Davengere, India

来源：

MOLECULAR BIOLOGY REPORTS | 2018年 / 45卷 / 05期

关键词：

Salacia; Genetic diversity; PCoA; Polymorphism; DNA POLYMORPHISM; AFLP MARKERS; MANGIFERIN; DIVERSITY; CULTIVARS;

D O I：

10.1007/s11033-018-4248-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diversity and phylogenetic relationship between four closely related Salacia species, i.e., Salacia chinensis, Salacia macrosperma, Salacia fruticosa and Salacia oblonga, collected from the Western Ghats of Karnataka, India, was assessed. Ten each of RAPD and ISSR primers generated a total of 76 and 68 loci, generating polymorphisms of 92.21 and 89.71%, respectively. Maximum likelihood analysis of the ITS sequences revealed three clades. Dendrogram analyses of RAPD and ISSR revealed two and four clusters, respectively. Overall polymorphism revealed by RAPD was 41.45 +/- 10%, ISSR was 33.58 +/- 6.52%, and ITS was 25.50 +/- 17.25%. Molecular variance revealed significant variance within and among the Salacia species. Tajima's D neutrality test and Fu's Fs were negative for all four species, implying presences of rare alleles and population expansion. Comparative study of RAPD, ISSR and ITS for Salacia species has given an insight into the efficiency of each technique in detecting diversity within and among the population sampled in the Western Ghats of Karnataka.

引用

页码：931 / 941

页数：11

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