Effect of canine mesenchymal stromal cells loaded with paclitaxel on growth of canine glioma and human glioblastoma cell lines

被引：18
|
作者：
Bonomi, A. [1 ]
Ghezzi, E. [2 ]
Pascucci, L. [3 ]
Aralla, M. [2 ]
Ceserani, V. [4 ]
Pettinari, L. [2 ]
Cocce, V. [1 ]
Guercio, A. [5 ]
Alessandri, G. [4 ]
Parati, E. [4 ]
Brini, A. T. [1 ,6 ]
Zeira, O. [2 ]
Pessina, A. [1 ]
机构：
[1] Univ Milan, Dept Biomed Surg & Dent Sci, Via Pascal 36, I-20133 Milan, Italy
[2] Vet Hosp San Michele, Via 1 Maggio 37, I-26838 Lodi, Italy
[3] Univ Perugia, Dept Vet Med, Via San Costanzo 4, I-06126 Perugia, Italy
[4] IRCCS, Neurol Inst C Besta, Cellular Neurobiol Lab, Dept Cerebrovasc Dis, Via Celoria 11, I-20133 Milan, Italy
[5] Ist Zooprofilatt Sperimentale Sicilia A Mirri, Area Diagnost Virol, Palermo, Italy
[6] IRCCS, Galeazzi Orthopaed Inst, Milan, Italy
来源：
VETERINARY JOURNAL | 2017年 / 223卷
关键词：
Canine; Drug delivery; Glioma; Mesenchymal stromal cells; Paclitaxel; STEM-CELLS; IN-VITRO; ADIPOSE-TISSUE; EFFICACY; DELIVERY; VEHICLES; TUMORS;
D O I：
10.1016/j.tvjl.2017.05.005
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
This study investigated whether canine mesenchymal stromal cells (cMSCs) are able to take up and release paclitaxel (PTX) in active form, and therefore whether they have potential as a tool for therapeutic delivery of this drug. cMSCs from bone marrow and adipose tissue were isolated, expanded and characterised phenotypically. cMSCs were loaded with PTX (cMSCs-PTX) and their capacity for release of PTX was determined by their effect on proliferation of cancer cells. cMSCs-PTX derived from bone marrow and adipose tissue were able to take up and then release active PTX. cMSCs-PTC inhibited proliferation of the canine glioma cell line J3T, and the human glioblastoma cell lines T98G and U87MG. The potential of canine cMSCs-PTX for treatment of canine gliomas should be investigated further. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：45 / 51
页数：7
相关论文
共 50 条
  • [1] Canine adipose-derived mesenchymal stromal cells inhibit the growth of canine hematologic malignancy cell lines
    Yasumura, Yuyo
    Teshima, Takahiro
    Nagashima, Tomokazu
    Michishita, Masaki
    Shigechika, Hiroki
    Taira, Yoshiaki
    Suzuki, Ryohei
    Matsumoto, Hirotaka
    REGENERATIVE THERAPY, 2025, 28 : 301 - 313
  • [2] Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro
    Kafarnik, Christiane
    McClellan, Alyce
    Dziasko, Marc
    Daniels, Julie T.
    Guest, Deborah J.
    STEM CELLS AND DEVELOPMENT, 2020, 29 (07) : 425 - 439
  • [3] Manufacturing Mesenchymal Stromal Cells for the Treatment of Osteoarthritis in Canine Patients: Challenges and Recommendations
    Ivanovska, Ana
    Wang, Mengyu
    Arshaghi, Tarlan Eslami
    Shaw, Georgina
    Alves, Joel
    Byrne, Andrew
    Butterworth, Steven
    Chandler, Russell
    Cuddy, Laura
    Dunne, James
    Guerin, Shane
    Harry, Rob
    McAlindan, Aidan
    Mullins, Ronan A.
    Barry, Frank
    FRONTIERS IN VETERINARY SCIENCE, 2022, 9
  • [4] Mesenchymal stromal cells loaded with Paclitaxel (PacliMES) a potential new therapeutic approach on mesothelioma
    Cocce, Valentina
    Bonelli, Mara
    La Monica, Silvia
    Alfieri, Roberta
    Doneda, Luisa
    Martegani, Eleonora
    Alessandri, Giulio
    Lagrasta, Costanza Annamaria
    Gianni, Aldo
    Sordi, Valeria
    Petrella, Francesco
    Roncoroni, Leda
    Paino, Francesca
    Pessina, Augusto
    BIOCHEMICAL PHARMACOLOGY, 2023, 214
  • [5] Mesenchymal stromal cells loaded with paclitaxel induce cytotoxic damage in glioblastoma brain xenografts
    Pacioni, Simone
    D'Alessandris, Quintino Giorgio
    Giannetti, Stefano
    Morgante, Liliana
    De Pascalis, Ivana
    Cocce, Valentina
    Bonomi, Arianna
    Pascucci, Luisa
    Alessandri, Giulio
    Pessina, Augusto
    Falchetti, Maria Laura
    Pallini, Roberto
    STEM CELL RESEARCH & THERAPY, 2015, 6
  • [6] Cell-mediated drug delivery by gingival interdental papilla mesenchymal stromal cells (GinPa-MSCs) loaded with paclitaxel
    Brini, Anna Teresa
    Cocce, Valentina
    Ferreira, Lorena M. Jose
    Giannasi, Chiara
    Cossellu, Gianguido
    Gianni, Aldo Bruno
    Angiero, Francesca
    Bonomi, Arianna
    Pascucci, Luisa
    Falchetti, Maria Laura
    Ciusani, Emilio
    Bondiolotti, Gianpietro
    Sisto, Francesca
    Alessandri, Giulio
    Pessina, Augusto
    Farronato, Giampietro
    EXPERT OPINION ON DRUG DELIVERY, 2016, 13 (06) : 789 - 798
  • [7] Canine Adipose-Derived Mesenchymal Stromal Cells Reduce Cell Viability and Migration of Metastatic Canine Oral Melanoma Cell Lines In Vitro
    Teng, Fwu Shing
    Lainetti, Patricia de Faria
    Franzoni, Mayara Simao
    Leis Filho, Antonio Fernando
    Gomes, Cristina de Oliveira Massoco Salles
    Laufer-Amorim, Renee
    Amorim, Rogerio Martins
    Fonseca-Alves, Carlos Eduardo
    VETERINARY SCIENCES, 2024, 11 (12)
  • [8] COMPARISON OF CULTURE REQUIREMENTS FOR CANINE AND HUMAN UMBILICAL CORDDERIVED MESENCHYMAL STROMAL CELLS
    Burdzinska, Anna
    Bhale, Tanushree
    Roszczyk, Aleksander
    Zep, Pawel
    Dabrowski, Filip
    Galanty, Marek
    TISSUE ENGINEERING PART A, 2023, 29 (11-12) : 1349 - 1350
  • [9] Automated Large-Scale Production of Paclitaxel Loaded Mesenchymal Stromal Cells for Cell Therapy Applications
    Lisini, Daniela
    Nava, Sara
    Frigerio, Simona
    Pogliani, Simona
    Maronati, Guido
    Marcianti, Angela
    Cocce, Valentina
    Bondiolotti, Gianpietro
    Cavicchini, Loredana
    Paino, Francesca
    Petrella, Francesco
    Alessandri, Giulio
    Parati, Eugenio A.
    Pessina, Augusto
    PHARMACEUTICS, 2020, 12 (05)
  • [10] A Protocol for the Isolation, Culture, and Cryopreservation of Umbilical Cord-Derived Canine Mesenchymal Stromal Cells: Role of Cell Attachment in Long-Term Maintenance
    Wright, Adrienne
    Snyder, Larry
    Knights, Kaori
    He, Hong
    Springer, Nora L.
    Lillich, James
    Weiss, Mark L.
    STEM CELLS AND DEVELOPMENT, 2020, 29 (11) : 695 - 713
12345