High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI

被引：9

作者：

Fallows, Thomas W. ^{[1
,2
]}

McGrath, Andrew J. ^{[3
,4
]}

Silva, Joana ^{[1
,2
]}

McAdams, Simon G. ^{[1
,5
]}

Marchesi, Andrea ^{[1
,2
]}

Tuna, Floriana ^{[1
,6
]}

Flitsch, Sabine L. ^{[1
,2
]}

Tilley, Richard D. ^{[3
,4
,7
]}

Webb, Simon J. ^{[1
,2
]}

机构：

[1] Univ Manchester, Sch Chem, Oxford Rd, Manchester M13 9PL, Lancs, England

[2] Univ Manchester, Manchester Inst Biotechnol, 131 Princess St, Manchester M1 7DN, Lancs, England

[3] Univ New South Wales, Sch Chem, Sydney, NSW, Australia

[4] Univ New South Wales, Australian Ctr NanoMed, Sydney, NSW, Australia

[5] Univ Manchester, Sch Mat, Oxford Rd, Manchester, Lancs, England

[6] Univ Manchester, Photon Sci Inst, Oxford Rd, Manchester M13 9PL, Lancs, England

[7] Univ New South Wales, Mark Wainwright Analyt Ctr, Electron Microscope Unit, Sydney, NSW, Australia

来源：

NANOSCALE ADVANCES | 2019年 / 1卷 / 09期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

IRON-OXIDE NANOPARTICLES; FE3O4; NANOPARTICLES; CONTRAST AGENTS; FUNCTIONALIZATION; ARRAYS; CELL; MAGNETOPHORESIS; MICROARRAYS; BEHAVIOR; DESIGN;

D O I：

10.1039/c9na00376b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

There is a need for biofunctionalised magnetic nanoparticles for many biomedical applications, including MRI contrast agents that have a range of surface properties and functional groups. A library of eleven adducts, each formed by condensing a reducing sugar with a catechol hydrazide, for nanoparticle functionalisation has been created using a high-throughput chemical synthesis methodology. The enzymatic transformation of an N-acetylglucosamine (GlcNAc) adduct into an N-acetyllactosamine adduct by beta-1,4-galactosyltransferase illustrates how chemoenzymatic methods could provide adducts bearing complex and expensive glycans. Superparamagnetic iron oxide nanoparticles (8 nm diameter, characterised by TEM, DLS and SQUID) were coated with these adducts and the magnetic resonance imaging (MRI) properties of GlcNAc-labelled nanoparticles were determined. This straightforward approach can produce a range of MRI contrast agents with a variety of biofunctionalised surfaces.

引用

页码：3597 / 3606

页数：10

共 77 条

[1] Ethylenediaminetetraacetic acid capped superparamagnetic iron oxide (Fe3O4) nanoparticles: A novel preparation method and characterization [J].