Autophosphorylation at Thr286 of the α calcium-calmodulin kinase II in LTP and learning

被引：855
作者：
Giese, KP
Fedorov, NB
Filipkowski, RK
Silva, AJ
机构：
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[2] M Nencki Inst Expt Biol, Dept Neurophysiol, PL-02093 Warsaw, Poland
来源：
SCIENCE | 1998年 / 279卷 / 5352期
关键词：
D O I：
10.1126/science.279.5352.870
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The calcium-calmodulin-dependent kinase II (CaMKII) is required for hippocampal longterm potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)dependent activity, CaMKII can undergo autophosphorylation, resulting in CaM-independent activity. A point mutation was introduced into the alpha CaMKII gene that blocked the autophosphorylation of threonine at position 286 (Thr(286)) Of this kinase without affecting its CaM-dependent activity, The mutant mice had no N-methyl-D-aspartate receptor-dependent LTP in the hippocampal CAI area and showed no spatial learning in the Morris water maze. Thus, the autophosphorylation of alpha CaMII at Thr(286) appears to be required for LTP and learning.
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页码：870 / 873
页数：4
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