Modified simultaneous saccharification and co-fermentation of DLC pretreated corn stover for high-titer cellulosic ethanol production without water washing or detoxifying pretreated biomass

被引：25
作者：
Yuan, Xinchuan [1 ]
Shen, Guannan [1 ]
Chen, Sitong [1 ]
Chen, Xiangxue [1 ]
Zhang, Chengcheng [1 ]
Liu, Shuangmei [1 ]
Jin, Mingjie [1 ]
机构：
[1] Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, 200 Xiaolingwei St, Nanjing 210094, Peoples R China
来源：
ENERGY | 2022年 / 247卷
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
DLC pretreatment; Cellulosic ethanol; Corn stover; Simultaneous sacchari fication and Co; Fermentation; FBE strategy; SACCHAROMYCES-CEREVISIAE; COMMERCIAL ENZYMES; DILUTE-ACID; XYLOSE; GRAVITY; SSCF; GLUCOSE; YEAST;
D O I：
10.1016/j.energy.2022.123488
中图分类号：
O414.1 [热力学];
学科分类号：
摘要：
Corn stover (CS) pretreated by DLC (Densifying Lignocellulosic biomass with acidic Chemicals) pre-treatment exhibited higher digestibility and much better fermentability compared to traditional pre-treatments. Nevertheless, the xylose fermentation, which was a key for lignocellulosic ethanol production, during SHCF (Separate Hydrolysis and Co-Fermentation) was unsatisfactory at high solid loadings. In this study, modified SSCF (Simultaneous Saccharification and Co-Fermentation) was adopted to promote xylose utilization and ethanol yield. SSCF conditions (temperature, inoculation size, nitrogen source addition) were investigated. A novel strategy "Fed-batch of DLC Biomass and Enzyme (FBE)" was developed for SSCF at 28%e35% solid loadings, which showed higher sugar conversion, higher xylose consumption and superior ethanol yield compared to SHCF and traditional SSCF. The highest ethanol titer of 74.6 g/L produced based on DLC pretreatment was achieved during FBE SSCF without water washing or detoxification of DLC-CS. (c) 2022 Elsevier Ltd. All rights reserved.
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页数：12
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