Biosurfactants of Lactobacillus helveticus for biodiversity inhibit the biofilm formation of Staphylococcus aureus and cell invasion

被引：24

作者：

Jiang, Xinpeng ^{[1
,2
]}

Yan, Xin ^{[1
]}

Gu, Shanshan ^{[1
]}

Yang, Yan ^{[1
]}

Zhao, Lili ^{[3
]}

He, Xinmiao ^{[2
]}

Chen, Hongyan ^{[3
]}

Ge, Junwei ^{[1
]}

Liu, Di ^{[2
]}

机构：

[1] Northeast Agr Univ, Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China

[2] Heilongjiang Acad Agr Sci, Key Lab Combining Farming & Anim Husb, Minist Agr, Anim Husb Res Inst, Harbin 150086, Heilongjiang, Peoples R China

[3] Chinese Acad Agr Sci, Heilongjiang Prov Key Lab Lab Anim & Comparat Med, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150069, Heilongjiang, Peoples R China

来源：

FUTURE MICROBIOLOGY | 2019年 / 14卷 / 13期

基金：

中国国家自然科学基金;

关键词：

adhesion; biofilm formation; biosurfactants; host cell; Lactobacillus helveticus; Staphylococcus aureus; ACIDOPHILUS-DERIVED BIOSURFACTANT; GENE-EXPRESSION; ADHESION; ANTIADHESIVE; SENSITIVITY; PREVENTION; PLANTARUM; VIRULENCE;

D O I：

10.2217/fmb-2018-0354

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: This study aimed to evaluate the differences of biosurfactants produced by two Lactobacillus helveticus strains against the biofilm formation of Staphylococcus aureus in vitro and in vivo. Materials & methods: Scanning electron microscopy, Real time-quantitative PCR (RT-qPCR) and cell assay were used to analyze the inhibiting effect of biosurfactants against biofilm formation. Results & conclusion: Results showed that the biosurfactants have anti-adhesive and inhibiting effects on biofilm formation in vivo and in vitro. The biofilm-formative genes and autoinducer-2 signaling regulated these characteristics, and the biosurfactant L. helveticus 27170 is better than that of 27058. Host cell adhesion and invasion results indicated that the biosurfactants L. helveticus prevented the S. aureus invading the host cell, which may be a new strategy to eliminate biofilms.

引用

页码：1133 / 1146

页数：14

共 56 条

[11] Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensing [J].