Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status

被引：179

作者：

Huszar, G ^{[1
]}

Ozenci, CC ^{[1
]}

Cayli, S ^{[1
]}

Zavaczki, Z ^{[1
]}

Hansch, E ^{[1
]}

Vigue, L ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Obstet & Gynecol, Sperm Physiol Lab, New Haven, CT 06510 USA

来源：

FERTILITY AND STERILITY | 2003年 / 79卷

关键词：

human sperm; hyaluronic acid-binding; maturity; HspA2; chromatin integrity; viability; acrosomal integrity;

D O I：

10.1016/S0015-0282(03)00402-3

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To test, both in semen and washed-sperm fractions, whether hyaluronic acid (HA) binding is restricted to sperm that have completed cellular maturation. Design: Comparisons of sperm in semen and in HA-bound sperm fractions. Setting: University-based diagnostic and research andrology laboratory. Patient(s): Semen samples originated in men being tested for infertility. Intervention(s): The attributes of sperm maturity were tested by immunocytochemistry with creatine kinase and HspA2 antisera (highlights cytoplasmic retention in diminished-maturity sperm), aniline blue chromatin staining (detects persistent histones), pisum sativum lectin staining (reveals acrosomal integrity), and the FertiLight viability kit (highlights viable and nonviable sperm). Result(s): All markers of sperm maturity and immaturity supported the hypothesis that HA-bound sperm are mature. Nonbinding sperm exhibited cytoplasmic and nuclear properties of diminished maturity. The acrosomal status of HA-bound sperm was either unreacted or slightly capacitated, but not acrosome reacted. Only viable sperm exhibited HA binding. Conclusion(s): Sperm that are able to bind to HA are mature and have completed the spermiogenetic processes of sperm plasma membrane remodeling, cytoplasmic extrusion, and nuclear histone-protamine replacement. Hyaluronic acid-bound sperm show unreacted acrosomes. These studies provide further insights into the relationship between spermiogenesis and sperm function.

引用

页码：1616 / 1624

页数：9

共 35 条

[21]

HUSZAR G, 1990, J ANDROL, V11, P40

[22]

HUSZAR G, 1990, FERTIL STERIL, V54, P1127

[23] SPERM CREATINE-PHOSPHOKINASE ACTIVITY AS A MEASURE OF SPERM QUALITY IN NORMOSPERMIC, VARIABLE SPERMIC, AND OLIGOSPERMIC MEN [J].

HUSZAR, G ;

VIGUE, L ;

CORRALES, M .

BIOLOGY OF REPRODUCTION, 1988, 38 (05) :1061-1066

[24]

KORNOVSKI BS, 1994, FERTIL STERIL, V61, P935

[25] FISH assessment of aneuploidy frequencies in mature and immature human spermatozoa classified by the absence or presence of cytoplasmic retention [J].

Kovanci, E ;

Kovacs, T ;

Moretti, E ;

Vigue, L ;

Bray-Ward, P ;

Ward, DC ;

Huszar, G .

HUMAN REPRODUCTION, 2001, 16 (06) :1209-1217

[26]

Lalwani S, 1996, MOL REPROD DEV, V43, P495, DOI 10.1002/(SICI)1098-2795(199604)43:4<495::AID-MRD12>3.0.CO

[27]

2-Z

[28]

Mortimer D., 1994, PRACTICAL LAB ANDROL, P209

[29] EVIDENCE FOR PRESENCE OF HYALURONAN-BINDING PROTEIN ON SPERMATOZOA AND ITS POSSIBLE INVOLVEMENT IN SPERM FUNCTION [J].

RANGANATHAN, S ;

GANGULY, AK ;

DATTA, K .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 1994, 38 (01) :69-76

[30] Hyaluronic acid substantially increases the retention of motility in cryopreserved/thawed human spermatozoa [J].

Sbracia, M ;

Grasso, J ;

Sayme, N ;

Stronk, J ;

Huszar, G .

HUMAN REPRODUCTION, 1997, 12 (09) :1949-1954

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