Single-Cell Profiling Reveals Sex, Lineage, and Regional Diversity in the Mouse Kidney

被引:269
作者
Ransick, Andrew [1 ]
Lindstrom, Nils O. [1 ]
Liu, Jing [1 ]
Zhu, Qin [2 ]
Guo, Jin-Jin [1 ]
Alvarado, Gregory F. [1 ]
Kim, Albert D. [1 ]
Black, Hannah G. [1 ]
Kim, Junhyong [3 ]
McMahon, Andrew P. [1 ]
机构
[1] Univ Southern Calif, Keck Sch Med, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Dept Stem Cell Biol & Regenerat Med, Los Angeles, CA 90089 USA
[2] Univ Penn, Biomed Grad Studies, Grad Program Genom & Computat Biol, 160 BRB 2-3,421 Curie Blvd, Philadelphia, PA 19104 USA
[3] Univ Penn, Dept Biol, 415 S Univ Ave, Philadelphia, PA 19104 USA
关键词
RNA-SEQ; NEPHRON PROGENITORS; GENDER-DIFFERENCES; NUMBER; TUBULE; SUSCEPTIBILITY; TRANSPORTERS; EXPRESSION; PROLACTIN; BURDEN;
D O I
10.1016/j.devcel.2019.10.005
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Chronic kidney disease affects 10% of the population with notable differences in ethnic and sex-related susceptibility to kidney injury and disease. Kidney dysfunction leads to significant morbidity and mortality and chronic disease in other organ systems. A mouse-organ-centered understanding underlies rapid progress in human disease modeling and cellular approaches to repair damaged systems. To enhance an understanding of the mammalian kidney, we combined anatomy-guided single-cell RNA sequencing of the adult male and female mouse kidney with in situ expression studies and cell lineage tracing. These studies reveal cell diversity and marked sex differences, distinct organization and cell composition of nephrons dependent on the time of nephron specification, and lineage convergence, in which contiguous functionally related cell types are specified from nephron and collecting system progenitor populations. A searchable database, Kidney Cell Explorer (https://cello.shinyapps.io/kidneycellexplorer/), enables gene-cell relationships to be viewed in the anatomical framework of the kidney.
引用
收藏
页码:399 / +
页数:22
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