Determination of cyclosporine a by high-performance liquid chromatography with aryl oxalate chemiluminescence detection

被引：0

作者：

Katayama, M

Taniguchi, H

Matsuda, Y

Akihama, S

Kaneko, S

Ishikawa, H

Aikawa, A

Sandou, M

Kuroda, Y

Nozawa, S

机构：

[1] Meiji Coll Pharm, Dept Analyt Chem, Setagaya Ku, Tokyo 154, Japan

[2] Meiji Coll Pharm, Dept Biochem, Setagaya Ku, Tokyo 154, Japan

[3] Ichikawa Gen Hosp, Tokyo Dent Coll, Dept Obstet & Gynecol, Tokyo, Japan

[4] Ichikawa Gen Hosp, Tokyo Dent Coll, Dept Urol, Tokyo, Japan

[5] Ohta Gen Hosp, Dept Urol, Fukushima, Japan

[6] Ohta Gen Hosp, Dept Pharm, Fukushima, Japan

[7] Keio Univ, Sch Med, Dept Obstet & Gynecol, Tokyo 108, Japan

来源：

ANALYTICAL LETTERS | 1998年 / 31卷 / 04期

关键词：

aryl oxalate chemiluminescence; cyclosporine A; CL-LC; whole blood; solid-phase extraction;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monitoring of cyclosporine A in 40 whole blood samples from renal transplantation patients by high performance liquid chromatography with aryl oxala,te-sulforhodamine 101 chemiluminescence detection is described. These data were compared with fluorescence polarized immunoassay (Cyclosporine-SP Dynapack(TM) using monoclonal antibody for whole blood), with good results (r = 0.88, y = 0.80 + 5.07). These values are the same as reported by high performance liquid chromatography with photometric detection. By using a solid phase extraction cartridge, cyclosporine A was analyzed within 30 min. The analysis time could be shortened to one tenth that previously reported. The proposed method has the same sensitivity as fluorescence polarized immunoassay.

引用

页码：621 / 629

页数：9

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