Application of thiolated gold nanoparticles for the enhancement of glucose oxidase activity

被引：223
作者：
Pandey, Pratibha
Singh, Surinder P.
Arya, Sunil K.
Gupta, Vinay
Datta, Monika
Singh, Sukhvir
Malhotra, Bansi D.
机构：
[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Univ Delhi, Dept Phys & Astrophys, Delhi 110007, India
[3] Univ Delhi, Dept Chem, Delhi 110007, India
来源：
LANGMUIR | 2007年 / 23卷 / 06期
关键词：
D O I：
10.1021/la062901c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Glucose oxidase (GO(x)) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5-8 nm) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis-Menton constant obtained for the immobilized (3.74 mM) GO(x) compared with that for the free (5.85 mM) GO(x) suggests significant enhancement in the activity of GO(x) attached to thiolated gold nanoparticles. The covalently immobilized GO(x) thiolated nanoparticles exhibit a response time of 30 s, a shelf life of more than 6 months, and improved tolerance to both pH and temperature.
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收藏
页码：3333 / 3337
页数：5
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