Molecular electroporation and the transduction of oligoarginines

被引:18
作者
Cahill, Kevin [1 ]
机构
[1] Univ New Mexico, Dept Phys & Astron, Biophys Grp, Albuquerque, NM 87131 USA
关键词
CELL-PENETRATING PEPTIDES; HUMAN IMMUNODEFICIENCY VIRUS; TAT-FUSION PROTEINS; HAMSTER OVARY CELLS; LIPID-MEMBRANES; PHOSPHATIDYLSERINE SYNTHASE; DEPENDENT TRANSLOCATION; ELECTRICAL BREAKDOWN; MAMMALIAN-CELLS; GENE-PRODUCT;
D O I
10.1088/1478-3975/7/1/016001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Certain short polycations, such as TAT and polyarginine, rapidly pass through the plasma membranes of mammalian cells by an unknown mechanism called transduction as well as by endocytosis and macropinocytosis. These cell-penetrating peptides (CPPs) promise to be medically useful when fused to biologically active peptides. I offer a simple model in which one or more CPPs and the phosphatidylserines of the inner leaflet form a kind of capacitor with a voltage in excess of about 200 mV, high enough to create a molecular electropore. The model is consistent with an empirical upper limit on the cargo peptide of 40-60 amino acids and with experimental data on how the transduction of a polyarginine-fluorophore into mouse C2C12 myoblasts depends on the number of arginines in the CPP and on the CPP concentration. The model makes three testable predictions.
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页数:14
相关论文
共 63 条
[1]  
ABIDOR IG, 1979, BIOELECTROCH BIOENER, V6, P37
[2]  
Alberts B., 2008, MOL BIOL CELL, P652
[3]  
ALBERTS B, 2008, MOL BIOL CELL, P624
[4]  
Alberts B., 2008, MOL BIOL CELL, P620
[5]  
[Anonymous], MOL BIOL CELL
[6]   Lipid translocation across the plasma membrane of mammalian cells [J].
Bevers, EM ;
Comfurius, P ;
Dekkers, DWC ;
Zwaal, RFA .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1999, 1439 (03) :317-330
[7]   Charge-dependent translocation of the Trojan peptide penetratin across lipid membranes [J].
Binder, H ;
Lindblom, G .
BIOPHYSICAL JOURNAL, 2003, 85 (02) :982-995
[8]   Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? [J].
Brogden, KA .
NATURE REVIEWS MICROBIOLOGY, 2005, 3 (03) :238-250
[9]  
Bulte Jeff W M, 2006, Methods Mol Med, V124, P419
[10]  
CAHILL K, FORTRAN 90 CODES THI