Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative

被引:19
作者
Chopra, Lipsy [1 ]
Singh, Gurdeep [1 ]
Jena, Kautilya Kumar [1 ]
Verma, Himanshu [1 ]
Sahoo, Debendra K. [1 ]
机构
[1] CSIR, Biochem Engn Res & Proc Dev Ctr, Inst Microbial Technol, Chandigarh 160036, India
关键词
Bacillus sonorensis; Sonorensin; Optimization; Volumetric oxygen transfer coefficient; Biopreservative; RECOMBINANT ASPERGILLUS-NIGER; BACTERIOCIN PRODUCTION; OPTIMIZATION; PH; PURIFICATION; FERMENTATION; TEMPERATURE; COMPLEX; STRAIN; MEDIA;
D O I
10.1016/j.biortech.2014.10.105
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Media composition and environmental conditions were optimized using statistical tools, Plackett Burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate Bacillus sonorensis MT93 showing broad spectrum of antimicrobial activity. Under optimized conditions, MT93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. As oxygen supply is a critical parameter controlling growth and product formation in aerobic bioprocesses and used as a parameter for bioprocess scale up, the effects of oxygen transfer, in terms of volumetric oxygen transfer coefficient (k(L)a), on production of sonorensin was investigated using optimized medium composition in a bioreactor. Studies on effectiveness of sonorensin against Staphylococcus aureus and Listeria monocytogenes in fruit juice and as a preservative in pasteurized milk demonstrated its potential as a biopreservative in fruit products and shelf life extender of the pasteurized milk. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:358 / 366
页数:9
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