Immune response and protection elicited by DNA immunisation against Taenia cysticercosis

被引：34
作者：
Wang, QM [1 ]
Sun, SH [1 ]
Hu, ZL [1 ]
Wu, D [1 ]
Wang, ZC [1 ]
机构：
[1] Second Mil Med Univ, Dept Med Genet, Shanghai 200433, Peoples R China
来源：
VACCINE | 2003年 / 21卷 / 15期
关键词：
DNA vaccine; cysticercosis; apoptosis;
D O I：
10.1016/S0264-410X(02)00661-8
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The study evaluated DNA vaccination in Taenia solium cysticercosis prevention by using cDNA of an antigen (cC1) from T solium metacestode. pcDNA3-cC1 DNA vaccine was constructed by inserting the cDNA into the eukaryotic expression plasmid pcDNA3. Positive expression of the pcDNA3-cC1 product was confirmed by its transfection into COS7 cell and enzyme-linked immunoabsorbent assay using serum of pigs infected with T solium metacestode. Immunisation of BALB/c mice with three injections of pcDNA3-cC1 induced antigen-specific immune responses of the Thl phenotype. Inoculation of new-born pigs induced protection against challenge with T solium by 73.3% reduction of the metacestode number. Antibodies elicited by DNA immunisation with pcDNA3-cC1 specifically reacted with native cC1 protein, which was mainly restricted to the cyst wall of T solium metacestode. Positive apoptosis signals were also detected in the cyst wall cells of metacestode slices from pigs immunised with pcDNA3-cC1 by TUNEL staining method. Those suggested that apoptosis played a role in protecting pigs immunised with pcDNA3-cC1 nucleic acid vaccine from pathogen challenge. (C) 2002 Elsevier Science Ltd. All rights reserved.
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收藏
页码：1672 / 1680
页数：9
相关论文
共 31 条
  • [1] Cai X, 2001, Southeast Asian J Trop Med Public Health, V32 Suppl 2, P105
  • [2] COTTEN M, 1994, GENE THER, V1, P239
  • [3] Cruz-Revilla C, 2000, J PARASITOL, V86, P67, DOI 10.1645/0022-3395(2000)086[0067:TCCPEA]2.0.CO
  • [4] 2
  • [5] DANKO I, 1994, GENE THER, V1, P114
  • [6] Vaccination with plasmid DNA expressing antigen from genomic or cDNA gene forms induces equivalent humoral immune responses
    Drew, DR
    Lightowlers, M
    Strugnell, RA
    [J]. VACCINE, 1999, 18 (7-8) : 692 - 702
  • [7] Vaccination against Taenia solium cysticercosis
    Flisser, A
    Lightowlers, MW
    [J]. MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2001, 96 (03): : 353 - 356
  • [8] CpG oligodeoxynucleotides and interleukin-12 improve the efficacy of Mycobacterium bovis BCG vaccination in mice challenged with M-tuberculosis
    Freidag, BL
    Melton, GB
    Collins, F
    Klinman, DM
    Cheever, A
    Stobie, L
    Suen, W
    Seder, RA
    [J]. INFECTION AND IMMUNITY, 2000, 68 (05) : 2948 - 2953
  • [9] Induction of mucosal protection against primary, heterologous simian immunodeficiency virus by a DNA vaccine
    Fuller, DH
    Rajakumar, PA
    Wilson, LA
    Trichel, AM
    Fuller, JT
    Shipley, T
    Wu, MS
    Weis, K
    Rinaldo, CR
    Haynes, JR
    Murphey-Corb, M
    [J]. JOURNAL OF VIROLOGY, 2002, 76 (07) : 3309 - 3317
  • [10] García G, 2001, J PARASITOL, V87, P582, DOI 10.1645/0022-3395(2001)087[0582:IROAIT]2.0.CO
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