Highly Sensitive Fluorescence Imaging of Zn2+ and Cu2+ in Living Cells with Signal Amplification Based on Functional DNA Self-Assembly

被引：61

作者：

Si, Haibin ^{[1
]}

Sheng, Renjie ^{[1
]}

Li, Qingling ^{[1
]}

Feng, Jie ^{[1
]}

Li, Lu ^{[1
]}

Tang, Bo ^{[1
]}

机构：

[1] Shandong Normal Univ, Minist Educ, Inst Mol & Nano Sci,Key Lab Mol & Nano Probes,Col, Collaborat Innovat Ctr Functionalized Probes Chem, Jinan 250014, Shandong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2018年 / 90卷 / 15期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

ATOMIC EMISSION-SPECTROMETRY; HYBRIDIZATION CHAIN-REACTION; PLASMA-MASS SPECTROMETRY; CATALYTIC BEACON SENSOR; IN-VITRO SELECTION; METAL-IONS; GOLD NANOPARTICLES; TITANIUM-DIOXIDE; DRINKING-WATER; GENERAL-METHOD;

D O I：

10.1021/acs.analchem.7b05268

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Intracellular trace Zn2+ and Cu2+ play important roles in the regulation of cell function. Considering the limitations of existing metal ion detection methods regarding sensitivity and applicability to living cells, an amplification strategy based on functional DNA self-assembly under DNAzyme catalysis to improve the sensitivity of intracellular Zn2+ and Cu2+ imaging is reported. In this process, metal ions as cofactor can activate the catalysis of DNAzyme to shear substrate chains, and each broken substrate chain can initiate consecutive hybridizations of hairpin probes (1-1x) labeled with fluorophore, which can reflect the information on a single metal ion with multiple fluorophores. The detection limit can reach nearly 80 pM and high-sensitivity fluorescence imaging of intracellular Zn2+ and Cu2+ can be achieved. The results are important for research on cell function regulation associated with trace Zn2+ and Cu2+. This approach is also a new way to improve the sensitivity of other trace metal ion imaging

引用

页码：8785 / 8792

页数：8

共 72 条

[1] Differentially Selective Chemosensor with Fluorescence Off-On Responses on Cu2+ and Zn2+ Ions in Aqueous Media and Applications in Pyrophosphate Sensing, Live Cell Imaging, and Cytotoxicity [J].

Anbu, Sellamuthu ;

Ravishankaran, Rajendran ;

Guedes da Silva, M. Fatima C. ;

Karande, Anjali A. ;

Pombeiro, Armando J. L. .

INORGANIC CHEMISTRY, 2014, 53 (13) :6655-6664

[2] Quantitative imaging of selenium, copper, and zinc in thin sections of biological tissues (Slugs-Genus arion) measured by laser ablation inductively coupled of plasma mass spectrometry [J].

Becker, J. S. ;

Matusch, A. ;

Depboylu, C. ;

Dobrowolska, J. ;

Zoriy, M. V. .

ANALYTICAL CHEMISTRY, 2007, 79 (16) :6074-6080

[3] Imaging of copper, zinc, and other elements in thin section of human brain samples (Hippocampus) by laser ablation inductively coupled plasma mass spectrometry [J].

Becker, JS ;

Zoriy, MV ;

Pickhardt, C ;

Palomero-Gallagher, N ;

Zilles, K .

ANALYTICAL CHEMISTRY, 2005, 77 (10) :3208-3216

[4] Biological inorganic and bioinorganic chemistry of neurodegeneration based on prion and Alzheimer diseases [J].

Brown, DR ;

Kozlowski, H .

DALTON TRANSACTIONS, 2004, (13) :1907-1917

[5] Ratiometric and near-infrared molecular probes for the detection and imaging of zinc ions [J].

Carol, Priya ;

Sreejith, Sivaramapanicker ;

Ajayaghosh, Ayyappanpillai .

CHEMISTRY-AN ASIAN JOURNAL, 2007, 2 (03) :338-348

[6] Mitochondrial and ER-Targeted eCALWY Probes Reveal High Levels of Free Zn2+ [J].

Chabosseau, Pauline ;

Tuncay, Erkan ;

Meur, Gargi ;

Bellomo, Elisa A. ;

Hessels, Anne ;

Hughes, Stephen ;

Johnson, Paul R. V. ;

Bugliani, Marco ;

Marchetti, Piero ;

Turan, Belma ;

Lyon, Alexander R. ;

Merkx, Maarten ;

Rutter, Guy A. .

ACS CHEMICAL BIOLOGY, 2014, 9 (09) :2111-2120

[7] α-Monoacylated and α,α′- and α,β′-Diacylated Dipyrrins as Highly Sensitive Fluorescence "Turn-on" Zn2+ Probes [J].

Ding, Yubin ;

Li, Xin ;

Li, Tong ;

Zhu, Weihong ;

Xie, Yongshu .

JOURNAL OF ORGANIC CHEMISTRY, 2013, 78 (11) :5328-5338

[8] Triggered amplification by hybridization chain reaction [J].

Dirks, RM ;

Pierce, NA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (43) :15275-15278

[9] A DNAzyme cascade for the amplified detection of Pb2+ ions or L-histidine [J].

Elbaz, Johann ;

Shlyahovsky, Bella ;

Willner, Itamar .

CHEMICAL COMMUNICATIONS, 2008, (13) :1569-1571

[10] Chemiluminescent and Chemiluminescence Resonance Energy Transfer (CRET) Detection of DNA, Metal Ions, and Aptamer-Substrate Complexes Using Hemin/G-Quadruplexes and CdSe/ZnS Quantum Dots [J].

Freeman, Ronit ;

Liu, Xiaoqing ;

Willner, Itamar .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (30) :11597-11604

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