Heat treatments involved during conversion of buffalo milk into skim milk powder by spray drying process (inlet air temperature : 180-200degreesC; outlet air temperature : about 95degreesC) reduced thiamin, riboflavin, vitamin B-6, folic acid and total vitamin C by 11.57, 9.70, 13.11, 16.18 and 18.62% respectively. During one year storage of milk powder (total solids, 96.21%) at 30degreesC in sealed polyethylene bags of 500 g capacity and wrapped in brown paper bags, losses of water-soluble vitamins continued to occur and the losses were minimum for riboflavin and maximum for vitamin C. At the end of 6 months storage of skim milk powder, losses of thiamin, riboflavin, vitamin B6, folic acid and vitamin C were 5.3, 2.7, 11.5, 24.0 and 28.7% respectively. After 12 months of storage, the losses were almost doubled for riboflavin, folic acid and vitamin C and tripled for thiamin and vitamin B-6 as compared to that of 6 months.