Development of an Intracellular Screen for New Compounds Able To Inhibit Mycobacterium tuberculosis Growth in Human Macrophages

被引：53

作者：

Sorrentino, Flavia ^{[1
,2
]}

Gonzalez del Rio, Ruben ^{[2
]}

Zheng, Xingji ^{[1
]}

Presa Matilla, Jesus ^{[2
]}

Torres Gomez, Pedro ^{[2
]}

Martinez Hoyos, Maria ^{[2
]}

Perez Herran, Maria Esther ^{[2
]}

Mendoza Losana, Alfonso ^{[2
]}

Av-Gay, Yossef ^{[1
]}

机构：

[1] Univ British Columbia, Fac Med, Dept Med, Div Infect Dis, Vancouver, BC, Canada

[2] GSK, Dis Dev World, Madrid, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2016年 / 60卷 / 01期

关键词：

DRUG SUSCEPTIBILITY; PYRAZINAMIDE; DISCOVERY; VIRULENCE; MEMBRANE; MODEL; ACID;

D O I：

10.1128/AAC.01920-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and valuable tool for the discovery of new antimycobacterial compounds.

引用

页码：640 / 645

页数：6

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