Analysis of Deoxyribonucleic Acid Interstrand Cross-links Induced by Nitrosourea with High Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

被引：9

作者：

Bai Bao-Qing ^{[1
]}

Zhao Li-Jiao ^{[1
]}

Zhong Ru-Gang ^{[1
]}

机构：

[1] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 38卷 / 04期

关键词：

Nitrosourea; Deoxyribonucleic acid interstrand cross-link; High performance liquid chromatography-electrospray ionization tandem mass spectrometry; IN-VITRO; DNA; DAMAGE; CELLS;

D O I：

10.3724/SP.J.1096.2010.00532

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA interstrand crosslink induced by nitrosoureas was investigated by high performance liquid chromatography-electrospray ion mass spectrometry(HPLC-ESI-MS/MS), with the synthesized 1-[N-(2'-deoxycytidyl)]-2-[N-(2'-deoxyguanosyl)] ethane (dG-dC) as standard. Calf thymus DNA was treated with 1,3-bis-(2-chloroethyl)-1-nitrosourea(BCNU). The DNA samples were denatured, enzymatically digested, centrifugal filtered, and then separated on a C-18 reverse column with ammonium acetate aqueous solution and acetonitrile as mobile phase. Samples were ionized by electrospray ionization(ESI) in positive mode. First, qualitative analysis was carried out by MS1 and MS2 full-scan. Then the content of dG-dC in DNA samples were quantified by monitoring the ion transition of m/z 521 -> 289 with selecting reaction monitoring mode. This research provided an effective method for the qualitative and quantitative analysis of DNA interstrand crosslink.

引用

页码：532 / 536

页数：5

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