Enhanced Effect of Combining Chlorogenic Acid on Selenium Nanoparticles in Inhibiting Amyloid β Aggregation and Reactive Oxygen Species Formation In Vitro

The deposition of amyloid-beta (A beta) plaques and formation of neurotoxic reactive oxygen species (ROS) is a significant pathological signature of Alzheimer's disease (AD). Herein, a novel strategy is reported for combining the unique A beta absorption property of selenium nanoparticles with the natural antioxidant agent chlorogenic acid (CGA) to form CGA@SeNPs. The in vitro biological evaluation revealed that CGA could clear the ROS induced by A beta 40 aggregates, but it did not inhibit the A beta 40 aggregation and cell membrane damage which were also caused by A beta 40 aggregates. Interestingly, CGA@SeNPs show an enhanced inhibition effect on A beta 40 aggregation and, more importantly, protect PC12 cells from A beta aggregation-induced cell death. It is believed that CGA@SeNPs are more efficient than CGA in reducing A beta 40 toxic in long-term use.