Human oral isolate Lactobacillus fermentum AGR1487 induces a proinflammatory response in germ-free rat colons

被引：16

作者：

Anderson, Rachel C. ^{[1
]}

Ulluwishewa, Dulantha ^{[1
,2
,6
]}

Young, Wayne ^{[1
]}

Ryan, Leigh J. ^{[1
]}

Henderson, Gemma ^{[3
]}

Meijerink, Marjolein ^{[4
]}

Maier, Eva ^{[1
,2
]}

Wells, Jerry M. ^{[4
]}

Roy, Nicole C. ^{[1
,2
,5
]}

机构：

[1] AgRes Grasslands, Food & Biobased Prod Grp, Food Nutr & Hlth Team, Palmerston North, New Zealand

[2] Massey Univ, Riddet Inst, Palmerston North, New Zealand

[3] AgRes Grasslands, Anim Hlth & Nutr Grp, Rumen Microbiol Team, Palmerston North, New Zealand

[4] Wageningen Univ, Anim Sci Grp, Host Microbe Interact, NL-6700 AP Wageningen, Netherlands

[5] Univ Auckland, Gravida Natl Ctr Growth & Dev, Auckland 1, New Zealand

[6] Curtin Univ, Sch Biomed Sci, Fac Hlth Sci, Bentley, WA 6102, Australia

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

P38; MAPK; MICE; PERMEABILITY; INFLAMMATION; HOMEOSTASIS; BACTEREMIA; IMMUNITY;

D O I：

10.1038/srep20318

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lactobacilli are thought to be beneficial for human health, with lactobacilli-associated infections being confined to immune-compromised individuals. However, Lactobacillus fermentum AGR1487 negatively affects barrier integrity in vitro so we hypothesized that it caused a pro-inflammatory response in the host. We compared germ-free rats inoculated with AGR1487 to those inoculated with another L. fermentum strain, AGR1485, which does not affect in vitro barrier integrity. We showed that rats inoculated with AGR1487 had more inflammatory cells in their colon, higher levels of inflammatory biomarkers, and increased colonic gene expression of pro-inflammatory pathways. In addition, our in vitro studies showed that AGR1487 had a greater capacity to activate TLR signaling and induce pro-inflammatory cytokines in immune cells. This study indicates the potential of strains of the same species to differentially elicit inflammatory responses in the host and highlights the importance of strain characterization in probiotic approaches to treat inflammatory disorders.

引用

页数：11

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