Efficacy of low concentrations of sodium hypochlorite and low-powered Er,Cr:YSGG laser activated irrigation against an Enterococcus faecalis biofilm

被引:34
作者
Christo, J. E. [1 ]
Zilm, P. S. [2 ]
Sullivan, T. [3 ]
Cathro, P. R. [1 ]
机构
[1] Univ Adelaide, Discipline Endodont, Adelaide, SA, Australia
[2] Univ Adelaide, Sch Dent, Oral Microbiol Lab, Adelaide, SA, Australia
[3] Univ Adelaide, Discipline Publ Hlth, Adelaide, SA, Australia
关键词
biofilm; endodontics; laser-activated irrigation; root canal disinfection; Sodium hypochlorite; INDUCED EXPLOSIVE VAPOR; ENDODONTIC TREATMENT; APICAL PERIODONTITIS; ROOT CANALS; SMEAR LAYER; TEETH; FLOW; IRRIGANTS; TIME; INSTRUMENTATION;
D O I
10.1111/iej.12447
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
AimTo establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without Er,Cr:YSGG laser activation on Enterococcus faecalis biofilms in extracted teeth. MethodologyThe root canals of 96 decoronated single-rooted extracted human teeth were prepared to a size 40, 0.06 taper 1mm beyond the apex. They were mounted within a flow cell, which was sterilized before pumping a nutrient media through the root canals. The flow cell was inoculated with E.faecalis (ATCC 700802) and cultivated for 4weeks. The root-ends were sealed, and the roots were then subjected to one of six treatment groups: group 1: syringe irrigation (SI) with saline (control) using a 27 -gauge Monoject needle 1mm from the apex for 2min; group 2: as for group 1 but with 1% NaOCl; group 3: as for group 1 but with 4% NaOCl; group 4: 0.5% NaOCl irrigation for 15s followed by laser-activated irrigation (LAI) with four 15-s cycles replenishing the irrigant between cycles; group 5: as for group 4 but with 1% NaOCl as the irrigant; group 6: as for group 4 but with 4% NaOCl as the irrigant. Following treatment, teeth were crushed and viable bacteria were quantitated by serial dilution and plating. The colony-forming unit values were compared between groups using one-way anova and Tukey-adjusted post hoc tests. A two-tailed P value of <0.05 was considered statistically significant. ResultsThe mean number of cells recovered from the 1% NaOCl SI group was significantly higher than that from the 4% NaOCl LAI group (P=0.02). ConclusionWithin the limitations of this laboratory study, low-powered (0.5W) Er,Cr:YSGG laser activation did not improve the antibacterial effect of low concentrations of sodium hypochlorite.
引用
收藏
页码:279 / 286
页数:8
相关论文
共 46 条
[21]   Visualization of Irrigant Flow and Cavitation Induced by Er:YAG Laser within a Root Canal Model [J].
Matsumoto, Himeka ;
Yoshimine, Yoshito ;
Akamine, Akifumi .
JOURNAL OF ENDODONTICS, 2011, 37 (06) :839-843
[22]   Microbiological status of root-filled teeth with apical periodontitis [J].
Molander, A ;
Reit, C ;
Dahlen, G ;
Kvist, T .
INTERNATIONAL ENDODONTIC JOURNAL, 1998, 31 (01) :1-7
[23]   Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment [J].
Nair, PNR ;
Henry, S ;
Cano, V ;
Vera, J .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 2005, 99 (02) :231-252
[24]   Biofilm removal by 6% sodium hypochlorite activated by different irrigation techniques [J].
Ordinola-Zapata, R. ;
Bramante, C. M. ;
Aprecio, R. M. ;
Handysides, R. ;
Jaramillo, D. E. .
INTERNATIONAL ENDODONTIC JOURNAL, 2014, 47 (07) :659-666
[25]   CYTO-TOXIC EFFECTS OF NAOCL ON VITAL TISSUE [J].
PASHLEY, EL ;
BIRDSONG, NL ;
BOWMAN, K ;
PASHLEY, DH .
JOURNAL OF ENDODONTICS, 1985, 11 (12) :525-528
[26]   Decontamination efficacy of photon-initiated photoacoustic streaming (PIPS) of irrigants using low-energy laser settings: an ex vivo study [J].
Pedulla, E. ;
Genovese, C. ;
Campagna, E. ;
Tempera, G. ;
Rapisarda, E. .
INTERNATIONAL ENDODONTIC JOURNAL, 2012, 45 (09) :865-870
[27]   Measurement of pressure changes during laser-activated irrigant by an erbium, chromium: yttrium, scandium, gallium, garnet laser [J].
Peeters, Harry Huiz ;
De Moor, Roeland J. G. .
LASERS IN MEDICAL SCIENCE, 2015, 30 (05) :1449-1455
[28]   Radiographic examination of apical extrusion of root canal irrigants during cavitation induced by Er,Cr:YSGG laser irradiation: an in vivo study [J].
Peeters, Harry Huiz ;
Mooduto, Latief .
CLINICAL ORAL INVESTIGATIONS, 2013, 17 (09) :2105-2112
[29]   THE FATE AND THE ROLE OF BACTERIA LEFT IN ROOT DENTINAL TUBULES [J].
PETERS, LB ;
WESSELINK, PR ;
MOORER, WR .
INTERNATIONAL ENDODONTIC JOURNAL, 1995, 28 (02) :95-99
[30]   Disinfection of Root Canals with Photon-initiated Photoacoustic Streaming [J].
Peters, Ove A. ;
Bardsley, Sean ;
Fong, Jennifer ;
Pandher, Goldie ;
DiVito, Enrico .
JOURNAL OF ENDODONTICS, 2011, 37 (07) :1008-1012