Polycomb Responds to Low Levels of Transcription

被引：16

作者：

Berrozpe, Georgina ^{[1
]}

Bryant, Gene O. ^{[1
]}

Warpinski, Katherine ^{[1
]}

Spagna, Dan ^{[1
]}

Narayan, Santosh ^{[1
]}

Shah, Shivangi ^{[1
]}

Ptashne, Mark ^{[1
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Mol Biol Program, 430 East 67th St, New York, NY 10065 USA

来源：

CELL REPORTS | 2017年 / 20卷 / 04期

关键词：

REPRESSIVE COMPLEX 2; NONCODING RNAS; DNA METHYLATION; GENOME-WIDE; CHROMATIN; GENE; BINDING; RECRUITMENT; COHESIN; LOCUS;

D O I：

10.1016/j.celrep.2017.06.076

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

How is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specificmammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells revealed that Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level and less so with promoters of genes that are either silent or more highly expressed. Studies of the CpG island promoter of the Kit gene demonstrate that Pc is largely absent when the gene is silent in myeloid cells, as well as when the gene is highly expressed in mast cells. Manipulations that increase transcription in the former case, and reduce it in the latter, increase Pc occupancy. The average negative effect of Pc, we infer, is about 2-fold. We suggest possible biological roles for such negative effects and propose a mechanism by which Pc might be recruited to weakly transcribed genes.

引用

页码：785 / 793

页数：9

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