Three-way multivariate calibration procedures applied to high-performance liquid chromatography coupled with fast-scanning fluorescence spectrometry detection.: Determination of polycyclic aromatic hydrocarbons in water samples

Three-way partial least-squares and n factor parallel factor analysis have been compared for the analysis of polycyclic aromatic hydrocarbons in water samples. Data were obtained with a chromatographic system set to record short-time chromatograms containing several unresolved peaks. The detection system consisted of a fast-scanning fluorescence spectra detector, which allows one to obtain three-dimensional data, where retention time, emission wavelengths, and fluorescence intensity are represented. The combined use of a multivariate calibration method and the three-dimensional data obtained from the HPLC-FSFS system allows resolution of closely eluting compounds, thus making a complete separation unnecessary. The procedure has been applied to tap water samples (spiked at 0.10 and 0.20 mu g L-1 levels) with good results, similar to those obtained with a HPLC system with a conventional fluorescence detector.