Trans-spliced mRNA products produced from circRNA expression vectors

被引：6
|
作者：
Chu, Jennifer [1 ,5 ]
Robert, Francis [1 ]
Pelletier, Jerry [1 ,2 ,3 ,4 ]
机构：
[1] McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada
[2] McGill Univ, Dept Oncol, Montreal, PQ H3A 1G5, Canada
[3] McGill Univ, Rosalind & Morris Goodman Canc Res Ctr, Montreal, PQ H3A 1A3, Canada
[4] McGill Univ, Ctr Rech Biol Struct, Montreal, PQ H3G 1Y6, Canada
[5] MIT, Dept Biol, Cambridge, MA 02138 USA
来源：
RNA | 2021年 / 27卷 / 06期
基金：
加拿大健康研究院;
关键词：
circRNA; trans-splicing; IRES; gene expression; CIRCULAR RNA; TRANSCRIPTION; TRANSLATION; BIOGENESIS; INITIATION; REGION;
D O I：
10.1261/rna.078261.120
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Circular (circ) RNA expression vectors are used as a method of identifying and characterizing RNA sequences that harbor internal ribosome entry site (IRES) activity. During the course of developing a vector series tailored for IRES discovery, we found evidence for the occurrence of trans-spliced mRNAs arising when sequences with promoter activity were embedded between the upstream CTD and downstream NTD exons of the pre-mRNA. These trans-spliced products regenerate the same open reading frame expected from a circRNA and can lead to false-positive signals in screens relying on circRNA expression vectors for IRES discovery. Our results caution against interpretations of IRES activity solely based on results obtained from circRNA expression vectors.
引用
收藏
页码：676 / 682
页数：7
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