A novel competence gene, comP, is essential for natural transformation of Acinetobacter sp. strain BD413

被引:0
作者
Porstendorfer, D [1 ]
Drotschmann, U [1 ]
Averhoff, B [1 ]
机构
[1] UNIV GOTTINGEN,INST MIKROBIOL,D-37077 GOTTINGEN,GERMANY
关键词
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Acinetobacter sp, strain BD413 (= ATCC 33305), a nutritionally versatile bacterium, has an extremely efficient natural transformation system, Here we describe the generation of eight transformation-affected mutants of Acinetobacter sp, strain BD413 by insertional mutagenesis. These mutants were found by Southern blot analysis and complementation studies to result from single nptII marker insertions at different chromosomal loci, DNA binding and uptake studies with one mutant, T205, revealed that the transformation deficiency of this mutant results from a complete lack of DNA binding and, therefore, uptake activity, A novel competence gene essential for natural transformation, named comP, was cloned by complementation of mutant T205, The nucleotide sequence of comP was determined, and its deduced 15-kDa polypeptide displays significant similarities to type IV pilins, Analysis of the ultrastructure of a transformation-deficient comP mutant and the transformation-competent wild-type strain revealed that both are covered with bundle-forming thin fimbriae (3 to 4 nm in diameter) and individual thick fimbriae (6 nm in diameter). These results provide evidence that the pilinlike ComP is unrelated to the piluslike structures of strain BD413. Taking all data into account, we propose that ComP functions as a major subunit of an organelle acting as a channel or pore mediating DNA binding and/or uptake in Acinetobacter sp, strain BD413.
引用
收藏
页码:4150 / 4157
页数:8
相关论文
共 46 条
[1]   COMPETENCE FOR GENETIC-TRANSFORMATION IN ACINETOBACTER-CALCOACETICUS-NCIB8250 [J].
AHLQUIST, EF ;
FEWSON, CA ;
RITCHIE, DA ;
PODMORE, J ;
ROWELL, V .
FEMS MICROBIOLOGY LETTERS, 1980, 7 (02) :107-109
[2]   NUCLEOTIDE-SEQUENCE AND GENETIC ORGANIZATION OF THE BACILLUS-SUBTILIS COMG OPERON [J].
ALBANO, M ;
BREITLING, R ;
DUBNAU, DA .
JOURNAL OF BACTERIOLOGY, 1989, 171 (10) :5386-5404
[3]   CLONING AND CHARACTERIZATION OF A CLUSTER OF LINKED BACILLUS-SUBTILIS LATE COMPETENCE MUTATIONS [J].
ALBANO, M ;
DUBNAU, DA .
JOURNAL OF BACTERIOLOGY, 1989, 171 (10) :5376-5385
[4]  
ANDERSON MLM, 1985, NUCLEIC ACID HYBRIDI, P73
[5]   REGULATION OF NITROGEN-FIXATION GENES [J].
AUSUBEL, FM .
CELL, 1984, 37 (01) :5-6
[6]   GENETIC-ANALYSIS OF SUPRAOPERONIC CLUSTERING BY USE OF NATURAL TRANSFORMATION IN ACINETOBACTER-CALCOACETICUS [J].
AVERHOFF, B ;
GREGGJOLLY, L ;
ELSEMORE, D ;
ORNSTON, LN .
JOURNAL OF BACTERIOLOGY, 1992, 174 (01) :200-204
[7]   A MEMBRANE-PROTEIN WITH SIMILARITY TO N-METHYLPHENYLALANINE PILINS IS ESSENTIAL FOR DNA-BINDING BY COMPETENT BACILLUS-SUBTILIS [J].
BREITLING, R ;
DUBNAU, D .
JOURNAL OF BACTERIOLOGY, 1990, 172 (03) :1499-1508
[8]  
BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376
[9]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[10]   CLONING AND GENETIC ORGANIZATION OF THE PCA GENE-CLUSTER FROM ACINETOBACTER-CALCOACETICUS [J].
DOTEN, RC ;
NGAI, KL ;
MITCHELL, DJ ;
ORNSTON, LN .
JOURNAL OF BACTERIOLOGY, 1987, 169 (07) :3168-3174