Centella asiatica (L.) urban from Nepal: Quali-quantitative analysis of samples from several sites, and selection of high terpene containing populations for cultivation

被引：45

作者：

Devkota, Anjana ^{[2
]}

Dall'Acqua, Stefano ^{[1
]}

Comai, Stefano ^{[1
]}

Innocenti, Gabbriella ^{[1
]}

Jha, Pramod Kumar ^{[2
]}

机构：

[1] Univ Padua, Dept Pharmaceut Sci, I-35131 Padua, Italy

[2] Tribhuvan Univ, Cent Dept Bot, Kathmandu, Nepal

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2010年 / 38卷 / 01期

关键词：

Centella asiatica; Asiaticoside; Triterpene; Quali-quantitative analysis; HPLC; TOTAL TRITERPENIC FRACTION; ALTITUDINAL VARIATION; ACTIVE CONSTITUENTS; DIVERSITY;

D O I：

10.1016/j.bse.2009.12.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Centella asiatica (L) Urban is widely used in traditional medicine in many countries and in the formulation of drugs and cosmetics, and is therefore suitable as a trade item for the development of medicinal plants for the population of Nepal. The aim of this work was to select plant populations of C asiatica with high contents of secondary metabolites growing in various localities in Nepal, and to enhance knowledge of the cultivation of this plant. Quali-quantitative analysis of bioactive triterpenes (asiaticoside and asiatic acid) and phenol derivatives (flavonoids and caffeoyl esters) was performed by HPLC-DAD-ELSD. The highest quantities of triterpenes and phenols were found in samples from the Gorkha and Chitwan districts. Regarding cultivated plants, soil fertilisation is critical, since over-rich soils affect secondary metabolite content. Plants growing in sand-rich soils produce more terpenes. This work provides indications on how to select high-terpene producing germplasm and recommendations for plant cultivation. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：12 / 22

页数：11

共 13 条

[1] Microcirculatory effects of total triterpenic fraction of Centella asiatica in chronic venous hypertension:: Measurement by laser Doppler, TcPO2-CO2, and leg volumetry [J].