Effects of saturated long-chain N-acylethanolamines on voltage-dependent Ca2+ fluxes in rabbit T-tubule membranes

The effects of saturated long-chain (C: 16-22) N-acylethanolamines and a series of saturated fatty acids with the same length of carbon chains were investigated on depolarization-induced Ca-45(2+) fluxes mediated by voltage-dependent Ca2+ channels in transverse tubule membrane vesicles from rabbit skeletal muscle. Vesicles were loaded with Ca-45(2+) and membrane potentials were generated by establishing potassium gradients across the vesicle using the ionophore valinomycin. Arachidonoylethanolamide and docosaenoylethanolamide but not palmitoylethanolamide and stearoylethanolamide (all 10 muM) caused a significant inhibition of depolarization-induced Ca-45(2+) fluxes and specific binding of [H-3]Isradipine to transverse tubule membranes. On the other hand. Saturated fatty acids including palmitic, stearic, arachidic, and docosanoic acids (all 10 muM) were ineffective in functional and radioligand binding experiments. Additional experiments using endocannabinoid metabolites suggested that whereas ethanolamine and arachidic acids were ineffective, arachidonoylethanolamide inhibited Ca2+ effluxes and specific binding of [H-3]Isradipine. Further studies indicated that only those fatty acids containing ethanolamine as a head group and having a chain length of more than 18 carbons were effective in inhibiting depolarization-induced Ca2+ effluxes and specific binding of [H-3]Isradipine. In conclusion, results indicate that depending on the chain length and the head group of fatty acid, N-acylethanolamines have differential effects on the function of voltage-dependent Ca2+ channels and on the specific binding of [H-3]Isradipine in skeletal Muscle membranes. (C) 2004 Elsevier Inc. All rights reserved.