Quantitative phase measurements of human cell nuclei using X-ray ptychography

被引：2

作者：

Schwenke, Jorg ^{[1
,2
,3
]}

Yusuf, Mohammed ^{[1
,2
,4
]}

Shemilt, Laura A. ^{[1
,2
]}

Wagner, Ulrich ^{[5
]}

Sajid, Atiqa ^{[4
]}

Morrison, Graeme R. ^{[1
,2
]}

Zhang, Fucai ^{[1
,2
]}

Parsons, Aaron ^{[5
]}

Rau, Christoph ^{[5
]}

Robinson, Ian K. ^{[1
,2
,6
]}

机构：

[1] UCL, London Ctr Nanotechnol, 17-19 Gordon St, London WC1H 0AH, England

[2] Rutherford Appleton Lab, Res Complex Harwell RCaH, Oxford OX11 0FA, England

[3] Lund Univ, MAX IV Lab, POB 118, SE-22100 Lund, Sweden

[4] Aga Khan Univ, Ctr Regenerat Med & Stem Cell Res, Karachi, Pakistan

[5] Diamond Light Source Ltd, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England

[6] Brookhaven Natl Lab, Condensed Matter Phys & Mat Dept, Upton, NY 11973 USA

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2021年 / 28卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

X-ray; ptychography; nuclei; quantitative phase imaging; synchrotron; HUMAN-CHROMOSOMES; DRY MASS; SHAPE; MICROSCOPY; DNA;

D O I：

10.1107/S1600577521004586

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.

引用

页码：1166 / 1173

页数：8

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