Comparison of the effects of nobiletin and letrozole on the activity and expression of aromatase in the MCF-7 breast cancer cell line

被引:12
|
作者
Rahideh, Seyedeh Tayebeh [1 ]
Keramatipour, Mohammad [2 ]
Nourbakhsh, Mitra [3 ]
Koohdani, Fariba [4 ]
Hoseini, Mostafa [5 ]
Talebi, Saeed [6 ]
Shidfar, Farzad [1 ]
机构
[1] Iran Univ Med Sci, Sch Publ Hlth, Dept Nutr, Tehran, Iran
[2] Univ Tehran Med Sci, Sch Med, Dept Med Genet, Tehran, Iran
[3] Iran Univ Med Sci, Sch Med, Dept Biochem, Tehran, Iran
[4] Univ Tehran Med Sci, Sch Nutr Sci & Dietet, Dept Mol & Cellular Nutr, Tehran, Iran
[5] Univ Tehran Med Sci, Sch Publ Hlth, Dept Epidemiol & Biostat, Tehran, Iran
[6] Iran Univ Med Sci, Sch Med, Dept Genet, Tehran, Iran
关键词
nobiletin; letrozole; gene expression; aromatase; breast cancer; ANTIPROLIFERATIVE ACTIVITY; RAT PLASMA; IN-VITRO; GROWTH; PHYTOESTROGENS; INHIBITORS; FLAVONOIDS; POLYMETHOXYFLAVONES; PROLIFERATION; HESPERETIN;
D O I
10.1139/bcb-2016-0206
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nobiletin (NOB) is one of the polymethoxyflavones mainly found in citrus fruits. Aromatase or cytochrome P450 (CYP19) enzyme catalyzes the last and rate-limiting step in estrogen biosynthesis. This study was carried out to investigate the effect of NOB on the activity and expression of aromatase, and to compare this property with letrozole (LET) as aromatase inhibitor in the MCF-7 breast cancer cell line. Cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays. Aromatase enzyme activity based on the conversion of androgenic substrate testosterone into 17 beta-estradiol was determined. CYP19 gene expression was measured by quantitative real-time PCR. MTT assays demonstrated that NOB at a concentration of 100 mu mol/L decreased cell viability in a time-dependent manner (P < 0.05). NOB significantly inhibited aromatase at the concentration of 0.1 mu mol/L (P = 0.013), whereas other concentrations had no effect. Treatment with 10 mu mol/L and 1 mu mol/L of NOB for 48 h significantly increased (P = 0.001) and decreased (P = 0.02) relative aromatase expression, respectively. The combination of LET and NOB had no effect on aromatase. This study showed for the first time that NOB decreases the activity and expression of aromatase at low concentrations in MCF-7 breast cancer cells.
引用
收藏
页码:468 / 473
页数:6
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