Isolation and Identification of Antioxidant Peptides from Black Soybean Protein

被引：0
作者：
Geng, Zhong-hua [1 ]
Liu, En-qi [1 ]
Zhang, Jian-ping [1 ]
Chen, Shang-long [1 ]
Li, Yong [1 ]
Wu, Yong-hua [1 ]
机构：
[1] Xuzhou Inst Technol, Jiangsu Key Lab Food Resource Dev & Qual Safety, Xuzhou 221008, Jiangsu, Peoples R China
来源：
MATERIALS, MACHINES AND DEVELOPMENT OF TECHNOLOGIES FOR INDUSTRIAL PRODUCTION | 2014年 / 618卷
关键词：
Peptides; Antioxidant; Structural analysis; DIGESTS;
D O I：
10.4028/www.scientific.net/AMM.618.298
中图分类号：
TH [机械、仪表工业];
学科分类号：
0802 ;
摘要：
The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates(BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods on Sephadex G-25 column and reversed phase high-performance liquid chromatography (RH-HPLC). Two highly purified antioxidant peptides SBP3 and BSPb were got, and their amino acid sequences were confirmed as Trp-Asn-Pro and Tyr-Asn-Ile by automated Edman degradation with a protein sequencer, respectively.
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页码：298 / 302
页数：5
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