High-throughput in vivo vertebrate screening

被引：0

作者：

Pardo-Martin, Carlos ^{[1
,2
,3
]}

Chang, Tsung-Yao ^{[1
]}

Koo, Bryan Kyo ^{[1
]}

Gilleland, Cody L. ^{[1
]}

Wasserman, Steven C. ^{[4
]}

Yanik, Mehmet Fatih ^{[1
,4
]}

机构：

[1] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[3] MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[4] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

NATURE METHODS | 2010年 / 7卷 / 08期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

ZEBRAFISH; DISEASE; MODEL;

D O I：

10.1038/NMETH.1481

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.

引用

页码：634 / U46

页数：5

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