Performances of the BD MAXTM CDIFF assay for the detection of toxigenic Clostridioides difficile using Cary-Blair preserved samples

被引：1

作者：

Anne-Gaelle, R. A. N. C. ^{[1
]}

Celia, S. E. N. T. I. S. ^{[1
]}

Jeanne, C. O. U. T. U. R. I. E. R. ^{[2
]}

Frederic, B. A. R. B. U. T. ^{[2
]}

Anne, T. R. I. S. T. A. N. ^{[1
]}

Coralie, B. U. I. S. ^{[1
]}

Kevin, S. A. N. T. O. S. ^{[1
]}

Sonia, F. A. R. R. A. H. ^{[1
]}

Pascaline, D. U. R. A. F. F. O. U. R. G. ^{[1
]}

Francois, V. A. N. D. E. N. E. S. C. H. ^{[1
]}

Olivier, D. A. U. W. A. L. D. E. R. ^{[1
]}

Frederic, L. A. U. R. E. N. T. ^{[1
]}

机构：

[1] Hosp Civils Lyon, Inst Infect Agents, Groupement Hosp Nord, Lyon, France

[2] Hop St Antoine, AP HP, Natl Reference Lab Clostridioides difficile, Paris, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2022年 / 103卷 / 03期

关键词：

Clostridioidesdifficile; diagnosis; molecular diagnosis; BD Max; Cary-Blair; BINARY TOXIN; LABORATORY DIAGNOSIS; EPIDEMIOLOGY; INFECTION; PCR; STRAINS; SOCIETY; UPDATE;

D O I：

10.1016/j.diagmicrobio.2022.115701

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Toxigenic Clostridioides difficile infection (CDI) is a high concern because of its increasing prevalence, among both nosocomial infection and community-acquired infection contexts. A total of 1320 prospective stool samples collected on FecalSwabTM (Cary-Blair medium) were screened using BD MaxTM CDIFF assay (BDM). Results were compared to the routine algorithm including immunochromatographic testing for glutamate dehydrogenase (GDH) and toxin A/B (tox) using C. DIFF QUIK CHEK COMPLETE (R) followed by Xpert C. difficile assay in case of GDH+/tox- results. The prevalence of toxigenic C. difficile was estimated to 5.3% (n=70/1320) using the routine algorithm but to 7.4% (n = 98/1320) using BDM. The overall agreement between BDM and the routine algorithm was 97.4%. After resolution of discrepant results between BDM and the algorithm using Xpert C. difficile assay (unless already perform) and toxigenic culture performed by the French National Reference Laboratory, the overall agreement was increased to 99.4%, demonstrating the excellent performances of the test.

引用

页数：5

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