Performances of the BD MAXTM CDIFF assay for the detection of toxigenic Clostridioides difficile using Cary-Blair preserved samples

被引：1

作者：

Anne-Gaelle, R. A. N. C. ^{[1
]}

Celia, S. E. N. T. I. S. ^{[1
]}

Jeanne, C. O. U. T. U. R. I. E. R. ^{[2
]}

Frederic, B. A. R. B. U. T. ^{[2
]}

Anne, T. R. I. S. T. A. N. ^{[1
]}

Coralie, B. U. I. S. ^{[1
]}

Kevin, S. A. N. T. O. S. ^{[1
]}

Sonia, F. A. R. R. A. H. ^{[1
]}

Pascaline, D. U. R. A. F. F. O. U. R. G. ^{[1
]}

Francois, V. A. N. D. E. N. E. S. C. H. ^{[1
]}

Olivier, D. A. U. W. A. L. D. E. R. ^{[1
]}

Frederic, L. A. U. R. E. N. T. ^{[1
]}

机构：

[1] Hosp Civils Lyon, Inst Infect Agents, Groupement Hosp Nord, Lyon, France

[2] Hop St Antoine, AP HP, Natl Reference Lab Clostridioides difficile, Paris, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2022年 / 103卷 / 03期

关键词：

Clostridioidesdifficile; diagnosis; molecular diagnosis; BD Max; Cary-Blair; BINARY TOXIN; LABORATORY DIAGNOSIS; EPIDEMIOLOGY; INFECTION; PCR; STRAINS; SOCIETY; UPDATE;

D O I：

10.1016/j.diagmicrobio.2022.115701

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Toxigenic Clostridioides difficile infection (CDI) is a high concern because of its increasing prevalence, among both nosocomial infection and community-acquired infection contexts. A total of 1320 prospective stool samples collected on FecalSwabTM (Cary-Blair medium) were screened using BD MaxTM CDIFF assay (BDM). Results were compared to the routine algorithm including immunochromatographic testing for glutamate dehydrogenase (GDH) and toxin A/B (tox) using C. DIFF QUIK CHEK COMPLETE (R) followed by Xpert C. difficile assay in case of GDH+/tox- results. The prevalence of toxigenic C. difficile was estimated to 5.3% (n=70/1320) using the routine algorithm but to 7.4% (n = 98/1320) using BDM. The overall agreement between BDM and the routine algorithm was 97.4%. After resolution of discrepant results between BDM and the algorithm using Xpert C. difficile assay (unless already perform) and toxigenic culture performed by the French National Reference Laboratory, the overall agreement was increased to 99.4%, demonstrating the excellent performances of the test.

引用

页数：5

共 17 条

[1]

Barbut Frederic, 2015, Presse Med, V44, pe75, DOI 10.1016/j.lpm.2014.09.021

[2] Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile [J].

Bidet, P ;

Lalande, V ;

Salauze, B ;

Burghoffer, B ;

Avesani, V ;

Delmée, M ;

Rossier, A ;

Barbut, F ;

Petit, JC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) :2484-2487

[3] Evaluation of three enzyme immunoassays and a loop-mediated isothermal amplification test for the laboratory diagnosis of Clostridium difficile infection [J].

Bruins, M. J. ;

Verbeek, E. ;

Wallinga, J. A. ;

van Coppenraet, L. E. S. Bruijnesteijn ;

Kuijper, E. J. ;

Bloembergen, P. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (11) :3035-3039

[4] Epidemiology of Community-Associated Clostridium difficile Infection, 2009 Through 2011 [J].

Chitnis, Amit S. ;

Holzbauer, Stacy M. ;

Belflower, Ruth M. ;

Winston, Lisa G. ;

Bamberg, Wendy M. ;

Lyons, Carol ;

Farley, Monica M. ;

Dumyati, Ghinwa K. ;

Wilson, Lucy E. ;

Beldavs, Zintars G. ;

Dunn, John R. ;

Gould, L. Hannah ;

MacCannell, Duncan R. ;

Gerding, Dale N. ;

McDonald, L. Clifford ;

Lessa, Fernanda C. .

JAMA INTERNAL MEDICINE, 2013, 173 (14) :1359-1367

[5] Nucleic Acid Amplification Test Quantitation as Predictor of Toxin Presence in Clostridium difficile Infection [J].

Crobach, M. J. T. ;

Duszenko, N. ;

Terveer, E. M. ;

Verduin, C. M. ;

Kuijper, E. J. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2018, 56 (03)

[6] European Society of Clinical Microbiology and Infectious Diseases: update of the diagnostic guidance document for Clostridium difficile infection [J].

Crobach, M. J. T. ;

Planche, T. ;

Eckert, C. ;

Barbut, F. ;

Terveer, E. M. ;

Dekkers, O. M. ;

Wilcox, M. H. ;

Kuijper, E. J. .

CLINICAL MICROBIOLOGY AND INFECTION, 2016, 22 :S63-S81

[7] Evolution of Testing Algorithms at a University Hospital for Detection of Clostridium difficile Infections [J].

Culbreath, Karissa ;

Ager, Edward ;

Nemeyer, Ronald J. ;

Kerr, Alan ;

Gilligan, Peter H. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (09) :3073-3076

[8] Current Trends in the Epidemiology and Outcomes of Clostridium difficile Infection [J].

Evans, Charlesnika T. ;

Safdar, Nasia .

CLINICAL INFECTIOUS DISEASES, 2015, 60 :S66-S71

[9] Frequency of binary toxin genes among Clostridium difcile strains that do not produce large clostridial toxins [J].

Geric, B ;

Johnson, S ;

Gerding, DN ;

Grabnar, M ;

Rupnik, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) :5227-5232

[10] Role of fecal Clostridium difficile load in discrepancies between toxin tests and PCR: is quantitation the next step in C-difficile testing? [J].

Leslie, J. L. ;

Cohen, S. H. ;

Solnick, J. V. ;

Polage, C. R. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (12) :3295-3299

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