Therapeutic Vaccine against Streptococcus sobrinus-induced Caries

被引：7
作者：
Dinis, M
Tavares, D
Fonseca, AJMM
Faria, R
Ribeiro, A
Cabrita, AMS
Ferreira, P
机构：
[1] ICBAS, Immunol Lab, P-40099003 Oporto, Portugal
[2] Inst Biol Mol & Celular, Oporto, Portugal
[3] Univ Coimbra, Fac Med, Coimbra Hosp, P-3000 Coimbra, Portugal
[4] Fac Med Coimbra, Inst Patol Expt, Coimbatore, Tamil Nadu, India
来源：
JOURNAL OF DENTAL RESEARCH | 2004年 / 83卷 / 04期
关键词：
Streptococcus sobrinus; dental caries; vaccination; VIP;
D O I：
10.1177/154405910408300416
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Streptococcus sobrinus produces a virulence-associated immunomodulatory protein (VIP) which suppresses the host-specific immune response and induces the early production of IL-10. In this study, we evaluated the effects of therapeutic immunization with this VIP on the incidence of caries in S. sobrinus-infected rats. Groups of Wistar rats were orally infected with S. sobrinus and fed with sucrose-sweetened drinking water ad libitum. Five days later, rats were immunized intranasally with active or heat-inactivated VIP plus alum as adjuvant or PBS plus adjuvant (sham-immunized). After 3 wks, all rats were re-immunized as above. Evaluation of dental caries showed that VIP-immunized animals had significantly fewer enamel sulcal and proximal caries lesions than did the sham-immunized animals (p < 0.001). The protective effects following therapeutic VIP immunization were attributed to the induced salivary immunoglobulin A specific to the VIP. These results offer a promising and safe strategy for the development of a vaccine against dental caries.
引用
收藏
页码：354 / 358
页数：5
相关论文
共 33 条
[1]   EVIDENCE FOR THE SYNTHESIS AND RELEASE OF STRONGLY IMMUNOSUPPRESSIVE, NON-CYTOTOXIC SUBSTANCES BY STREPTOCOCCUS-INTERMEDIUS [J].
ARALACHAVES, MP ;
HIGERD, TB ;
PORTO, MT ;
MUNOZ, J ;
GOUST, JM ;
FUDENBERG, HH ;
LOADHOLT, CB .
JOURNAL OF CLINICAL INVESTIGATION, 1979, 64 (04) :871-883
[2]   IS PROPHYLACTIC IMMUNOSTIMULATION OF THE HOST AGAINST PATHOGENIC MICROBIAL ANTIGENS AN ADEQUATE STRATEGY OF IMMUNOPROTECTION [J].
ARALACHAVES, MP .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1992, 35 (05) :495-500
[3]  
ARALACHAVES MP, 1988, AM J VET RES, V49, P1955
[4]  
BLANDER SJ, 1991, J IMMUNOL, V147, P285
[5]  
Caufield P W, 1997, Pediatr Dent, V19, P491
[6]  
Coykendall A. L., 1986, MOL MICROBIOLOGY IMM, P21
[7]   DIFFERENCES IN CARIOGENICITY BETWEEN FRESH ISOLATES OF STREPTOCOCCUS-SOBRINUS AND STREPTOCOCCUS-MUTANS [J].
DESOET, JJ ;
VANLOVEREN, C ;
LAMMENS, AJ ;
PAVICIC, MJAMP ;
HOMBURG, CHE ;
TENCATE, JM ;
DEGRAAFF, J .
CARIES RESEARCH, 1991, 25 (02) :116-122
[8]   Purification, and biochemical and biological characterization of an immunosuppressive and lymphocyte mitogenic protein secreted by Streptococcus sobrinus [J].
Ferreira, P ;
Bras, A ;
Tavares, D ;
Vilanova, M ;
Ribeiro, A ;
Videira, A ;
AralaChaves, M .
INTERNATIONAL IMMUNOLOGY, 1997, 9 (11) :1735-1743
[9]   CORRELATION BETWEEN SPECIFIC IMMUNOSUPPRESSION AND POLYCLONAL B-CELL ACTIVATION INDUCED BY A PROTEIN SECRETED BY STREPTOCOCCUS-MUTANS [J].
FERREIRA, P ;
SOARES, R ;
RIBEIRO, A ;
ARALACHAVES, M .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1988, 27 (05) :549-554
[10]   Current status of a mucosal vaccine against dental caries [J].
Hajishengallis, G ;
Michalek, SM .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1999, 14 (01) :1-20
1234