Partial protective responses induced by a recombinant cysteine proteinase from Leishmania (Leishmania) amazonensis in a murine model of cutaneous leishmaniasis

被引：8
作者：
Cardoso Fedeli, Carlos Eduardo
Lima Ferreira, Josie Haydee [2 ]
Mussalem, Juliana Sekeres
Longo-Maugeri, Ieda Maria
Gentil, Luciana Girotto
Machado dos Santos, Marcia Regina [3 ]
Katz, Simone
Barbieri, Clara Lucia [1 ]
机构：
[1] Univ Fed Sao Paulo, Div Parasitol, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, Brazil
[2] Univ Fed Piaui, Picos, Piaui, Brazil
[3] Univ Bandeirante Sao Paulo, Sao Paulo, Brazil
来源：
EXPERIMENTAL PARASITOLOGY | 2010年 / 124卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
Leishmania (Leishmania) amazonensis; Cutaneous leishmaniasis; Cysteine proteinase; Immune response; T-CELL RESPONSE; VISCERAL LEISHMANIASIS; IMMUNE-RESPONSES; INFECTED MACROPHAGES; BALB/C MICE; ANTIGEN; DNA; VACCINATION; INFANTUM; AMASTIGOTES;
D O I：
10.1016/j.exppara.2009.09.003
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A 500 bp fragment encoding an isoform of cysteine proteinase from Leishmania (Leishmania) amazonensis was subcloned and expressed in the pHis vector, resulting in a recombinant protein of 24 kDa, rLacys24. In Western blots of L (L) amazonensis extracts, antibodies directed to rLacys24 recognized a cysteine proteinase isoform of 30 kDa. Analysis by fluorescence-activated cell sorter showed a significantly higher expression of CD8(+) lymphocytes in animals immunized with rLacys24 plus CFA, whereas a low expression of CD4(+) lymphocytes was observed in these animals. The cytotoxicity of lymphocytes isolated from mice immunized with rLacys24 plus CFA on L. (L.) amazonensis-infected macrophages was significantly higher than that observed in the presence of lymphocytes from control animals. Immunization of BALB/c mice with rLacys24 plus CFA resulted in a low but significant decrease of foot lesions after challenge with L (L) amazonensis compared to those exhibited by control mice. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：153 / 158
页数：6
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