Ohmic heating as a method of obtaining paraprobiotics: Impacts on cell structure and viability by flow cytometry

被引：36
作者：
Barros, Cassia P. [1 ]
Pires, Roberto P. S. [2 ]
Guimaraes, Jonas T. [1 ]
Abud, Yuri K. D. [3 ]
Almada, Caroline N. [4 ]
Pimentel, Tatiana C. [5 ]
Sant'Anna, Celso [3 ]
De-Melo, Luiz Dione B. [2 ]
Duarte, Maria Carmela K. H. [1 ]
Silva, Marcia C. [2 ]
Sant'Ana, Anderson S. [4 ]
Freitas, Monica Q. [1 ]
Cruz, Adriano G. [2 ]
机构：
[1] Fluminense Fed Univ, Fac Vet, Dept Food Technol, Niteroi, RJ, Brazil
[2] Fed Inst Sci & Technol Rio de Janeiro, Dept Food, Rio De Janeiro, RJ, Brazil
[3] Natl Inst Metrol Qual & Technol, Duque De Caxias, RJ, Brazil
[4] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Campinas, SP, Brazil
[5] Fed Inst Parana, Paranavai, PR, Brazil
来源：
FOOD RESEARCH INTERNATIONAL | 2021年 / 140卷
关键词：
Inactivated probiotics; Ghost probiotics; Cell membrane damage; Emerging technology; Cell morphology;
D O I：
10.1016/j.foodres.2020.110061
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This study aimed to evaluate the effects of ohmic heating (OH) on probiotic inactivation, cell viability and morphology of the probiotic strains Lactobacillus acidophilus LA 05 (LA), Lacticaseibacillus casei 01 (LC), and Bifidobacterium animalis Bb 12 (BA) to develop paraprobiotics. OH at different electric field magnitudes (4, 8, and 12 V/cm at 60 Hz) and conventional heat treatment (CONV) were performed to determine the most adequate condition for the obtainment of paraprobiotics. Analysis of culturability, flow cytometry (FC), and Scanning electron microscope (SEM) was carried out. The complete inactivation by CONV was achieved only in the following conditions: LA -95 degrees C/5 min, LC and BA -95 degrees C/7 min. The same temperature profile was used in OH treatments to study the OH electrical effects. The OH treatment (8 V/cm) caused lower damage to the cell membrane integrity compared to the CONV treatment (p < 0.05). The OH showed to be adequate technology for the efficient production of paraprobiotics.
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页数：11
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共 49 条
  • [1] Microwave-freeze drying of lactic acid bacteria: Influence of process parameters on drying behavior and viability
    Ambros, S.
    Mayer, R.
    Schumann, B.
    Kulozik, U.
    [J]. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, 2018, 48 : 90 - 98
  • [2] Comparison of inactivation pathways of thermal or high pressure inactivated Lactobacillus rhamnosus ATCC 53103 by flow cytometry analysis
    Ananta, E.
    Knorr, D.
    [J]. FOOD MICROBIOLOGY, 2009, 26 (05) : 542 - 546
  • [3] Influence of stressing conditions caused by organic acids and salts on tolerance of Listeria monocytogenes to Origanum vulgare L. and Rosmarinus officinalis L. essential oils and damage in bacterial physiological functions
    Barbosa, Isabella de Medeiros
    da Cruz Almeida, Erika Tayse
    Cancado Castellano, Lucio Roberto
    de Souza, Evandro Leite
    [J]. FOOD MICROBIOLOGY, 2019, 84
  • [4] Barros C. P., 2020, FOOD RES INT
  • [5] Paraprobiotics and postbiotics: concepts and potential applications in dairy products
    Barros, Cassia P.
    Guimaraes, Jonas T.
    Esmerino, Erick A.
    Duarte, Maria Carmela K. H.
    Silva, Marcia C.
    Silva, Ramon
    Ferreira, Beatriz M.
    Sant'Ana, Anderson S.
    Freitas, Monica Q.
    Cruz, Adriano G.
    [J]. CURRENT OPINION IN FOOD SCIENCE, 2020, 32 : 1 - 8
  • [6] Ohmic heating in dairy processing: Relevant aspects for safety and quality
    Cappato, L. P.
    Ferreira, M. V. S.
    Guimaraes, J. T.
    Portela, J. B.
    Costa, A. L. R.
    Freitas, M. Q.
    Cunha, R. L.
    Oliveira, C. A. F.
    Mercali, G. D.
    Marzack, L. D. F.
    Cruz, A. G.
    [J]. TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2017, 62 : 104 - 112
  • [7] Ohmic Heating: A potential technology for sweet whey processing
    Costa, Naiara R.
    Cappato, Leandro P.
    Ferreira, Marcus Vinicius S.
    Pires, Roberto P. S.
    Moraes, Jeremias
    Esmerino, Erick A.
    Silva, Ramon
    Neto, Roberto P. C.
    Tavares, Maria Ines B.
    Freitas, Monica Q.
    Silveira Junior, Raimundo N.
    Rodrigues, Flavio N.
    Bisaggio, Rodrigo C.
    Cavalcanti, Rodrigo N.
    Raices, Renata S. L.
    Silva, Marcia C.
    Cruz, Adriano G.
    [J]. FOOD RESEARCH INTERNATIONAL, 2018, 106 : 771 - 779
  • [8] Mentha piperita L. essential oil inactivates spoilage yeasts in fruit juices through the perturbation of different physiological functions in yeast cells
    da Cruz Almeida, Erika Tayse
    de Souza, Geany Targino
    de Sousa Guedes, Jossana Pereira
    Barbosa, Isabella Medeiros
    de Sousa, Cristina Paiva
    Cancado Castellano, Lucio Roberto
    Magnani, Marciane
    de Souza, Evandro Leite
    [J]. FOOD MICROBIOLOGY, 2019, 82 : 20 - 29
  • [9] Investigation of damage to Escherichia coli, Listeria monocytogenes and Salmonella Enteritidis exposed to Mentha arvensis L. and M. piperita L. essential oils in pineapple and mango juice by flow cytometry
    de Sousa Guedes, Jossana Pereira
    de Souza, Evandro Leite
    [J]. FOOD MICROBIOLOGY, 2018, 76 : 564 - 571
  • [10] Application of flow cytometry to industrial microbial bioprocesses
    Diaz, Mario
    Herrero, Monica
    Garcia, Luis A.
    Quiros, Covadonga
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2010, 48 (03) : 385 - 407
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