Application of new phenolic antioxidants for cryopreservation of sturgeon sperm

被引:27
作者
Osipova, V. P. [1 ]
Berberova, N. T. [2 ]
Gazzaeva, R. A. [3 ]
Kudryavtsev, K. V. [4 ]
机构
[1] Russian Acad Sci, Southern Sci Ctr, St Chehova 41, Rostov Na Donu 344006, Russia
[2] Astrakhan State Tech Univ, Ul Tatishcheva 16, Astrakhan 414025, Russia
[3] North Ossetian State Univ, Vladikavkaz 362040, Russia
[4] Moscow MV Lomonosov State Univ, Leninskie Gory 1-3, Moscow 119991, Russia
基金
俄罗斯基础研究基金会;
关键词
Antioxidant; Lipid peroxidation; The sperm of the Russian sturgeon; Cryopreservation; Sterically hindered phenol; BUTYLATED HYDROXYTOLUENE BHT; VITRO FERTILIZING ABILITY; HUSO-HUSO SPERMATOZOA; ALPHA-TOCOPHEROL; LIPID-PEROXIDATION; BOAR SEMEN; ASCORBIC-ACID; RAM SEMEN; MOTILITY; CONSERVATION;
D O I
10.1016/j.cryobiol.2016.02.006
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects of BHT and water-soluble analogue of vitamin E, trolox. The most efficient antioxidant has the effective concentration of 0.1 mM. Novel antioxidant agents as cryomedium supplements not only reduced the level of lipid peroxidation, but also enhanced the translational motility of the sperm of the Russian sturgeon after defrosting. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:112 / 118
页数:7
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