Electron cryo-microscopy structure of the mechanotransduction channel NOMPC

被引:159
|
作者
Jin, Peng [1 ]
Bulkley, David [2 ]
Guo, Yanmeng [1 ]
Zhang, Wei [1 ,3 ]
Guo, Zhenhao [1 ]
Huynh, Walter [4 ]
Wu, Shenping [3 ]
Meltzer, Shan [1 ]
Cheng, Tong [1 ,3 ]
Jan, Lily Yeh [1 ,2 ,3 ]
Jan, Yuh-Nung [1 ,2 ,3 ]
Cheng, Yifan [2 ,3 ]
机构
[1] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94158 USA
[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
[3] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA
[4] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94158 USA
关键词
TRP CHANNEL; ION-CHANNEL; CRYSTAL-STRUCTURE; ANKYRIN REPEATS; ACTIVATION; VALIDATION; MICROSCOPY; DYNACTIN; FORCE; HELIX;
D O I
10.1038/nature22981
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mechanosensory transduction for senses such as proprioception, touch, balance, acceleration, hearing and pain relies on mechanotransduction channels, which convert mechanical stimuli into electrical signals in specialized sensory cells(1). How force gates mechanotransduction channels is a central question in the field, for which there are two major models. One is the membrane-tension model: force applied to the membrane generates a change in membrane tension that is sufficient to gate the channel, as in the bacterial MscL channel and certain eukaryotic potassium channels(2-5). The other is the tether model: force is transmitted via a tether to gate the channel. The transient receptor potential (TRP) channel NOMPC is important for mechanosensation-related behaviours such as locomotion, touch and sound sensation across different species including Caenorhabditis elegans(6), Drosophila(7-9) and zebrafish(10). NOMPC is the founding member of the TRPN subfamily(11), and is thought to be gated by tethering of its ankyrin repeat domain to microtubules of the cytoskeleton(12-15). Thus, a goal of studying NOMPC is to reveal the underlying mechanism of force-induced gating, which could serve as a paradigm of the tether model. NOMPC fulfils all the criteria that apply to mechanotransduction channels(1,7) and has 29 ankyrin repeats, the largest number among TRP channels. A key question is how the long ankyrin repeat domain is organized as a tether that can trigger channel gating. Here we present a de novo atomic structure of Drosophila NOMPC determined by single-particle electron cryo-microscopy. Structural analysis suggests that the ankyrin repeat domain of NOMPC resembles a helical spring, suggesting its role of linking mechanical displacement of the cytoskeleton to the opening of the channel. The NOMPC architecture underscores the basis of translating mechanical force into an electrical signal within a cell.
引用
收藏
页码:118 / +
页数:17
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